Protein Info for PFR28_00412 in Pseudomonas sp. RS175

Annotation: Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00513: acetyl-CoA carboxylase, carboxyl transferase, alpha subunit
  • amino acids 2 to 314 (313 residues), 509.2 bits, E=2.5e-157
• PF03255: ACCA
  • amino acids 5 to 148 (144 residues), 213.8 bits, E=9e-68
• PF01039: Carboxyl_trans
  • amino acids 91 to 245 (155 residues), 61.8 bits, E=5e-21

Best Hits

Swiss-Prot: 97% identical to ACCA_PSEPF: Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01962, acetyl-CoA carboxylase carboxyl transferase subunit alpha [EC: 6.4.1.2] (inferred from 98% identity to pba:PSEBR_a1081)

MetaCyc: 68% identical to acetyl-CoA carboxyltransferase subunit alpha (Escherichia coli K-12 substr. MG1655)
RXN0-5055 [EC: 2.1.3.15]

Predicted SEED Role

"Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.4.1.2)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type II) (3/3 steps found)
• superpathway of fatty acid biosynthesis initiation (4/5 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• propanoyl CoA degradation I (1/3 steps found)
• 2-oxobutanoate degradation I (1/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (4/10 steps found)
• candicidin biosynthesis (1/6 steps found)
• jadomycin biosynthesis (3/9 steps found)
• superpathway of candicidin biosynthesis (4/11 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (8/17 steps found)
• 3-hydroxypropanoate cycle (5/13 steps found)
• methylaspartate cycle (9/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (8/18 steps found)
• glyoxylate assimilation (4/13 steps found)
• superpathway of L-methionine salvage and degradation (6/16 steps found)
• superpathway of the 3-hydroxypropanoate cycle (5/18 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.2

Use Curated BLAST to search for 2.1.3.15 or 6.4.1.2

Protein Sequence (315 amino acids)

>PFR28_00412 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (Pseudomonas sp. RS175)
MNPNFLDFEQPIADLQAKIEELRLVGNDNSLNIGDEISRLQDKSRTLTEDIFGKLTSWQI
ARLARHPRRPYTLDYIEHIFTEFDELHGDRHFADDAAIVGGVARLEDKPVMVIGHQKGRE
VREKVRRNFGMPRPEGYRKACRLMEMAERFKMPILTFIDTPGAYPGIDAEERNQSEAIAW
NLRVMARLKTPIIATVIGEGGSGGALAIGVCDQLNMLQYSTYAVISPEGCASILWKTAEK
APDAAEAMGITAERLKGLGIVDKVINEPVGGAHRDPAAAAALIRAELSSQLAMLKKFDND
ALLARRYERLMSYGL