Protein Info for PFR28_00077 in Pseudomonas sp. RS175

Annotation: Carbamoyl-phosphate synthase large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 28 to 1079 (1052 residues), 1576.1 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 154 to 360 (207 residues), 243.8 bits, E=6.2e-76
  • amino acids 699 to 901 (203 residues), 122.3 bits, E=9.3e-39
• PF02787: CPSase_L_D3
  • amino acids 453 to 531 (79 residues), 87.9 bits, 1.7e-28
• PF02222: ATP-grasp
  • amino acids 708 to 871 (164 residues), 44.5 bits, E=5.9e-15
• PF02655: ATP-grasp_3
  • amino acids 708 to 871 (164 residues), 36 bits, E=3.3e-12
• PF07478: Dala_Dala_lig_C
  • amino acids 724 to 869 (146 residues), 29.6 bits, E=2.1e-10
• PF02142: MGS
  • amino acids 985 to 1067 (83 residues), 78.9 bits, 1.2e-25

Best Hits

Swiss-Prot: 75% identical to CARB_ECO57: Carbamoyl-phosphate synthase large chain (carB) from Escherichia coli O157:H7

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 78% identity to abo:ABO_0318)

MetaCyc: 76% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1099 amino acids)

>PFR28_00077 Carbamoyl-phosphate synthase large chain (Pseudomonas sp. RS175)
MRRPLRALQRLFDGLADRPADLSDKPMPKRTDIKSILILGAGPIVIGQACEFDYSGAQAC
KALREEGYRVILVNSNPATIMTDPAMADATYIEPIKWQTVAKIIEKERPDALLPTMGGQT
ALNCALDLEREGVLEKFGVEMIGANADTIDKAEDRSRFDKAMKSIGLECPRSGIAHSMEE
ANAVLERLGFPCIIRPSFTMGGTGGGIAYNREEFEEICSRGLDLSPTKELLIDESLIGWK
EYEMEVVRDKKDNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQILRNASLAVLRE
IGVETGGSNVQFGICPNTGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
LSNDITGGKTPASFEPSIDYVVTKLPRFAFEKFAKADARLTTQMKSVGEVMAIGRTFQES
LQKALRGLEVGVCGLDPKVDLNNPESMSELKRELTVPGAERIWYVADAFRAGMTVEQIFG
MNMIDPWFLVQIEDLVKEEEKVKTLGMSSIDRDTMFRLKRKGFSDMRLAKLLGVTEKSLR
AHRHKLEVFPVYKRVDTCAAEFATDTAYLYSTYEEECEAAPSGRDKIMILGGGPNRIGQG
IEFDYCCVHAALALRDDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLEDVLEIVRVEKP
KGVIVQYGGQTPLKLARALEAAGVPIIGTSPDAIDRAEDRERFQQMVERLNLRQPPNATV
RSEDEAIRAAAKIGYPLVVRPSYVLGGRAMEIVYQEEELKRYLREAVQVSNDSPVLLDHF
LNCAIEMDVDAVCDGKDVVIGAIMQHIEQAGVHSGDSACSLPPYSLPAHLQDEMREQVKK
MALELGVVGLMNVQLALQGEDIYVIEVNPRASRTVPFVSKCIGVSLAMIAARVMAGKTLA
ELNFTKEIIPNFYSVKEAVFPFAKFPGVDPILGPEMKSTGEVMGVGDTFGEAFAKAQMGA
SEVLPTGGTAFISVRNDDKPLVAGVARDLINLGFEVVATAGTAKVIEAAGLKVRRVNKVT
EGRPHVVDMIKNDEVTLIINTTEGRQSIADSYSIRRNALQHKIYCTTTIAAGEAICEALK
FGPEKTVRRLQDLHAGLKA