Protein Info for PFLU_RS28980 in Pseudomonas fluorescens SBW25

Annotation: ribosome-associated ATPase/putative transporter RbbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 905 transmembrane" amino acids 553 to 574 (22 residues), see Phobius details amino acids 708 to 733 (26 residues), see Phobius details amino acids 760 to 781 (22 residues), see Phobius details amino acids 790 to 813 (24 residues), see Phobius details amino acids 818 to 840 (23 residues), see Phobius details amino acids 847 to 869 (23 residues), see Phobius details amino acids 878 to 900 (23 residues), see Phobius details PF00005: ABC_tran" amino acids 23 to 169 (147 residues), 95.9 bits, see alignment E=8.6e-31 amino acids 284 to 428 (145 residues), 97.8 bits, see alignment E=2.2e-31 PF13304: AAA_21" amino acids 136 to 193 (58 residues), 32.8 bits, see alignment 1.9e-11 amino acids 359 to 458 (100 residues), 36.7 bits, see alignment E=1.2e-12 PF12679: ABC2_membrane_2" amino acids 538 to 836 (299 residues), 47.8 bits, see alignment E=3.1e-16 PF12698: ABC2_membrane_3" amino acids 557 to 897 (341 residues), 157.6 bits, see alignment E=1.2e-49 PF01061: ABC2_membrane" amino acids 706 to 867 (162 residues), 62 bits, see alignment E=1.5e-20

Best Hits

Swiss-Prot: 56% identical to RBBA_ECOLI: Ribosome-associated ATPase (rbbA) from Escherichia coli (strain K12)

KEGG orthology group: K13926, ribosome-dependent ATPase (inferred from 100% identity to pfs:PFLU5889)

Predicted SEED Role

"ABC-type multidrug transport system, permease component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3X9 at UniProt or InterPro

Protein Sequence (905 amino acids)

>PFLU_RS28980 ribosome-associated ATPase/putative transporter RbbA (Pseudomonas fluorescens SBW25)
MTALALQATGINHRYGKQQALVDITFSLPAGTRCGLIGPDGAGKSSLLGLIAGVKKLQDG
QLHVLGGAIEDRRHRNSLYPRIAFMPQGLGGNLYPELSIRENIRFFATLFGLSKADCDQR
MHNLLLATDLARFADRPAGKLSGGMKQKLGLCCALIHDPDLLILDEPTTGVDPLSRRRFW
ELVDSVRSERPQLTLLVATAYMEEAEQFEHCLMLDRGKLIADGLSSELAGATSSGKLDEA
FTHFQGDSAHDNQPLVIPPRESDNTDIAIEAHDLTLRFGDFTAVNNVSLAIGRGEIFGFL
GSNGCGKTTTMKVLTGLMPATEGSATLLGNPVNAKDLATRKRVGFMSQSFSLYGELSVRQ
NLVLHAQLFDLPTADSGPRIDELIQRFDLIDVAEQPSGELPLGLRQRLSLAVAVLHRPEV
LILDEPTSGVDPAARDDFWRLLIELSREQGVTIFLSTHFMNEAQRCDRISLMHAGKVLAC
DTPEALRRQFHGDTLEAAFVTCLEQAQGEAEPAAATATVSATSAPPVSKRGFSLARLLAV
ASREGKELLRDKVRMAFALLGAMFMMVIFGYGISLDVENLAFAVYDQDQTPQSRAYLEAF
RSSRYFAEQSPIQDSAELHRRLQRSEIKLALEIPPGFGRDLYAGRQPTVAAWLDGGMPFR
AETSRNYVEAVHQGNLAQLAELSSLPRNNLTAARLETRFRYNQDVVSVNAIGPGVMALIL
AFIPAMLTALGIVREKELGSITNFYATPLTRLEFLLGKQAPYLAVSLVNLAVLVAMNRWL
FGVPFKGSGLTLAFGGLLYVLATTSMGLLISAFTRTQIAAILGTMIITSLPTIQFSGLIV
PRSSLEGAAALMGMLFPAGHFLDITVGTFTKALDLRQLWPQCLALFGFFIGFTGLSLIML
KKQEA