Protein Info for PFLU_RS28595 in Pseudomonas fluorescens SBW25

Annotation: putative 2-aminoethylphosphonate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR03261: putative 2-aminoethylphosphonate ABC transporter, periplasmic 2-aminoethylphosphonate-binding protein" amino acids 6 to 335 (330 residues), 540.2 bits, see alignment E=8.9e-167 PF13531: SBP_bac_11" amino acids 25 to 281 (257 residues), 60.4 bits, see alignment E=4.8e-20 PF01547: SBP_bac_1" amino acids 32 to 277 (246 residues), 67.9 bits, see alignment E=3.2e-22 PF13416: SBP_bac_8" amino acids 40 to 278 (239 residues), 79.2 bits, see alignment E=9.1e-26 PF13343: SBP_bac_6" amino acids 76 to 307 (232 residues), 88.6 bits, see alignment E=9.5e-29

Best Hits

KEGG orthology group: K02012, iron(III) transport system substrate-binding protein (inferred from 100% identity to pfs:PFLU5809)

Predicted SEED Role

"Ferric iron ABC transporter, iron-binding protein" in subsystem Campylobacter Iron Metabolism or Iron acquisition in Vibrio or Transport of Iron

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3Q2 at UniProt or InterPro

Protein Sequence (340 amino acids)

>PFLU_RS28595 putative 2-aminoethylphosphonate ABC transporter substrate-binding protein (Pseudomonas fluorescens SBW25)
MFKPLALVAAVLTAFSLNAFAKTELTVYTALEAEQLKTYKQAFEKANPDIEIKWVRDSTG
IITAKLLAEKARPQADVVWGLAASSLAILDQQGMLDNYAPKDLDKIGKNYRDAANPPAWV
GMDVWAATICFNTVEAEKQGLTKPVSWQDLTKPEYKGKIVMPNPASSGTGFLDVSAWLQT
FGEKQGWQYMDDLHQNIGQYVHSGSKPCKLAASGEFPIGISFEYPAVQLKRQGAPLDIIL
PKEGLGWDIEATAVIKGTAHADAAKKLADFSASPAAMDLYKDNFAVLAQPGIAKPQTELP
ADYEQRLIKNDFAWASKNRDSILTEWRKRYDGKSEKVAGQ