Protein Info for PFLU_RS24655 in Pseudomonas fluorescens SBW25

Annotation: phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 837 PF00358: PTS_EIIA_1" amino acids 9 to 132 (124 residues), 146.8 bits, see alignment E=7.1e-47 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 10 to 130 (121 residues), 131.3 bits, see alignment E=2.8e-42 TIGR01003: phosphocarrier, HPr family" amino acids 172 to 251 (80 residues), 72.2 bits, see alignment E=4e-24 PF00381: PTS-HPr" amino acids 173 to 252 (80 residues), 81.1 bits, see alignment E=1.2e-26 PF05524: PEP-utilisers_N" amino acids 279 to 398 (120 residues), 75.3 bits, see alignment E=1.2e-24 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 279 to 828 (550 residues), 461.3 bits, see alignment E=6.6e-142 PF00391: PEP-utilizers" amino acids 423 to 495 (73 residues), 64.6 bits, see alignment E=1.3e-21 PF02896: PEP-utilizers_C" amino acids 521 to 806 (286 residues), 339.6 bits, see alignment E=3.8e-105

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 100% identity to pfs:PFLU5027)

Predicted SEED Role

"PTS system, glucose-specific IIA component (EC 2.7.1.69) / Phosphotransferase system, phosphocarrier protein HPr / Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Trehalose Uptake and Utilization or Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.1.69, EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K1I6 at UniProt or InterPro

Protein Sequence (837 amino acids)

>PFLU_RS24655 phosphoenolpyruvate--protein phosphotransferase (Pseudomonas fluorescens SBW25)
MSNNNNDLTLCAPLSGPVLTLGNVPDDVFASGAMGDGIAIDPLNDCLHAPFDGEIIHVAR
TGHALTIRAKNGAEVLMHVGIDTVELNGEGFALLVKNGARVSQGQALVQFDLDRIARQCK
SLVSLIILTNGERFELRALAGRTVKVGEPLLQIVARSAASAETPVDNSAGEASASVRITH
RGGLHARPAALVRKAAQGFSSQAQLHFGDKSASCDSLIGLMGLGIGEGDEVRVSCRGKDA
EAALQALVAALSAVIKEEHHAPAAAAAPRRANTEVGVLQGVCAAPGLVCGPLFRLTGIEL
PADTGNHSADEQLQRLDAALEQVRSEIRSTLDHARQRKNTEEEDIFAAHLALLEDPNLLE
AATGSIEQGSAATHAWRDAIQAQCAVLLALGKPLFAERANDLRDLQQRVLRALLGEAWHF
ELPAGAIVSAHELTPSDLLQLSAQHAVGLCMAEGGATSHVAILARGKGLPCVVALGADVL
DVPQGQRVVLDAANGRLELAPSDARHAEVHQIRDAQKLRRQQQQAQAQQPAHTTDGVSIE
VAANVASSAEAQVAFENGADGVGLLRTEFLFVDRRTAPDEQEQRHAYQAVLDAMGDKSVI
IRTIDVGGDKQLDYLPLPVEANPVLGLRGIRLAQVRPDVLDQQLRALLQVSPLARCRILL
PMVSEVDELLHIRQRLDELCVELELTQRPELGVMIEVPAAALMAEQLARYADFLSIGTND
LSQYTLAMDRDHAGLAARVDALHPALLRLIAQTCAGAAKHGRWVGVCGALASDPLATPVL
VGLGVSELSVSPLQIGEIKDRVRHLDAAQCRQLSQGLLDLSSAKAVRQACQHHWPLS