Protein Info for PFLU_RS24275 in Pseudomonas fluorescens SBW25

Annotation: sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 302 to 321 (20 residues), see Phobius details PF02518: HATPase_c" amino acids 473 to 580 (108 residues), 79.5 bits, see alignment E=1.3e-26

Best Hits

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 100% identity to pfs:PFLU4953)

Predicted SEED Role

"C4-dicarboxylate transport sensor protein"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K166 at UniProt or InterPro

Protein Sequence (588 amino acids)

>PFLU_RS24275 sensor histidine kinase (Pseudomonas fluorescens SBW25)
MIVIPRPLRLTFYSLLIIAGAVLAAALATRHAERQALVDDAARANQQLALYANSLHTLIE
RYRALPAVLALDSEMINALKGPLDPSTQDLLNRKLERINGAAQSSTLELMDRTGLAVAAS
NWNLPSSYVGHNYAFRPYFSQTLSQGTGRFYAVGVTTGIPGYFLSSAVLDEHEQFLGAMV
VKLEFPELEREWAQGNDLLLVSDARGIVFIANQPGWRYRNLRPLSASDLAELKATRQYDK
KQLLPLDAQSLQRFGENSHLMRVNGPEGSANYIWESLPLKAEGWTLHLLRKPQFAFEDQR
NAGLAAAGSWLALVFLVLFLTQRWRLARLRQRSREELEQLVEERTQALRTAQDGLVQSAK
LAALGQMSAALAHEINQPLTAQRMQLATLRLLLDHGRVDDAYKALTPLDDMLTRMAALTG
HLKTFARKSPSGLRERLDLATVVDQSLHLLDARLRDEAIGVVLDLTRPAWVRGDAIRLEQ
VLINLLRNALDAMADKPRKRLEIRLHADQQLWQLTVSDSGGGIADDHLNSVFDPFFTTKP
VGDGLGLGLAVSYAIVHELGGRLSVGNRGDGAVFTLTLPIALETPEPC