Protein Info for PFLU_RS23030 in Pseudomonas fluorescens SBW25

Annotation: dTMP kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00041: dTMP kinase
  • amino acids 2 to 197 (196 residues), 182.4 bits, E=4e-58
• PF02223: Thymidylate_kin
  • amino acids 8 to 197 (190 residues), 176.7 bits, E=4.2e-56

Best Hits

Swiss-Prot: 100% identical to KTHY_PSEFS: Thymidylate kinase (tmk) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00943, dTMP kinase [EC: 2.7.4.9] (inferred from 100% identity to pfs:PFLU4700)

MetaCyc: 49% identical to Tmk (Escherichia coli K-12 substr. MG1655)
T(2)-induced deoxynucleotide kinase. [EC: 2.7.4.12, 2.7.4.13, 2.7.4.9]

Predicted SEED Role

"Thymidylate kinase (EC 2.7.4.9)" (EC 2.7.4.9)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (5/9 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.12 or 2.7.4.13 or 2.7.4.9

Sequence Analysis Tools

See C3K0M9 at UniProt or InterPro

Protein Sequence (210 amino acids)

>PFLU_RS23030 dTMP kinase (Pseudomonas fluorescens SBW25)
MTGLFITLEGPEGAGKSTNRDYLAERLRSEGIEVVLTREPGGTPLAERIREVLLAPGEEQ
MNPDTELLLVFAARAQHLAEVIRPALARGAVVICDRFTDSTYAYQGGGRGLSLARIATLE
TFVQGDLRPDLTLVFDLPVEVGLARASARGRLDRFELEGQAFFDAVRTAFLTRAAAEPER
YYLLDAAQPLAHVQQAIDALLPTLLERARG