Protein Info for PFLU_RS22760 in Pseudomonas fluorescens SBW25

Annotation: 3-oxoacyl-ACP reductase FabG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 9 to 198 (190 residues), 182.6 bits, E=9.2e-58
• PF08659: KR
  • amino acids 12 to 119 (108 residues), 36.8 bits, E=5.8e-13
• PF13561: adh_short_C2
  • amino acids 18 to 247 (230 residues), 207.2 bits, E=4.4e-65

Best Hits

Swiss-Prot: 36% identical to GNO_GLUOX: Gluconate 5-dehydrogenase (gno) from Gluconobacter oxydans (strain 621H)

KEGG orthology group: K00059, 3-oxoacyl-[acyl-carrier protein] reductase [EC: 1.1.1.100] (inferred from 100% identity to pfs:PFLU4646)

MetaCyc: 44% identical to A-factor type gamma-butyrolactone 6-reductase (6R-forming) (Streptomyces coelicolor A3(2))
1.1.1.eb [EC: 1.1.1.eb]; 1.1.1.eb [EC: 1.1.1.eb]; 1.1.1.eb [EC: 1.1.1.eb]; 1.1.1.eb [EC: 1.1.1.eb]

Predicted SEED Role

"Oxidoreductase, short chain dehydrogenase/reductase family" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (18/20 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• biotin biosynthesis I (13/15 steps found)
• (5Z)-dodecenoate biosynthesis I (6/6 steps found)
• (5Z)-dodecenoate biosynthesis II (6/6 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• fatty acid elongation -- saturated (4/5 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
• palmitate biosynthesis III (21/29 steps found)
• stearate biosynthesis IV (4/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• IM-2 type γ-butyrolactones biosynthesis (2/5 steps found)
• petroselinate biosynthesis (2/6 steps found)
• 2-allylmalonyl-CoA biosynthesis (2/8 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (20/205 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.100

Use Curated BLAST to search for 1.1.1.100 or 1.1.1.eb

Sequence Analysis Tools

See C3K150 at UniProt or InterPro

Protein Sequence (248 amino acids)

>PFLU_RS22760 3-oxoacyl-ACP reductase FabG (Pseudomonas fluorescens SBW25)
MTTQNLSGKVALIQGGSRGIGAAIVKRLAAQGAAVAFTYVSSAAKADALQNSVISEGGKA
LAIQADSADAAAIRNAVNATVEAFGRIDILVNNAGVLAIAPLEDFKLEDFDQTLAINVRS
VFIATQEAAKHMGEGGRVINIGSTNAERMPFGGGGPYAMSKAALVGLTKGLARDLGPRGI
TINNVQPGPVDTDMNPANSDFAESLIGLMAVGRYGHVEEIASFVAYLAGPEAGYITGASL
TIDGGFSA