Protein Info for PFLU_RS21185 in Pseudomonas fluorescens SBW25

Annotation: polyphosphate:AMP phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 TIGR03708: polyphosphate:AMP phosphotransferase" amino acids 2 to 491 (490 residues), 735.8 bits, see alignment E=3.5e-225 PF03976: PPK2" amino acids 11 to 234 (224 residues), 171.6 bits, see alignment E=1.1e-54 amino acids 269 to 491 (223 residues), 317.3 bits, see alignment E=2.9e-99

Best Hits

Swiss-Prot: 81% identical to PK22_PSESM: Polyphosphate:AMP phosphotransferase (PSPTO_1640) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU4317)

Predicted SEED Role

"UDP-galactose-lipid carrier transferase (EC 2.-.-.-)" (EC 2.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K065 at UniProt or InterPro

Protein Sequence (504 amino acids)

>PFLU_RS21185 polyphosphate:AMP phosphotransferase (Pseudomonas fluorescens SBW25)
MFESAEIGHAIDKDTFEAEVPALREALLEVQYELQQQKRFPVIILINGIEGAGKGETVKL
LNEWMDPRLIEVRTFDQQTDEELARPPAWRYWRQLPAKGRMGIFFGNWYSQMLQGRVHGQ
IKDARLDQAIAGAERLEKMLCDEGALIFKFWFHLSKKQMKARLKALADDPLHSWRISPLD
WQQSETYDKFVHFGERVLRRTSRDYAPWHVIEGVDSNYRSLTVGKILLEGLQNALKLPKG
KASGMNPAPLPSAVDQMSLLNSLDMTLHLDKDDYEEQLITEQARFSGLMRDKRMRKHALV
TVFEGNDAAGKGGAIRRVAAALDPRQYHIVPIAAPTEDERAQPYLWRFWQKIPARGKFTV
FDRSWYGRVLVERIEGFCTPADWLRAYGEINDFEEQLRDAGVIVVKFWLAIDKQTQLERF
QAREEIPFKRFKITEDDWRNRDKWDDYRAAVGDMVDRTSTEVAPWTLVEANDKRWARVKV
LRTINLALEEAFAKSDKHDKKKKK