Protein Info for PFLU_RS18275 in Pseudomonas fluorescens SBW25

Annotation: sigma-54-dependent Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 603 PF00158: Sigma54_activat" amino acids 312 to 473 (162 residues), 222.5 bits, see alignment E=5.4e-70 PF14532: Sigma54_activ_2" amino acids 319 to 478 (160 residues), 64.5 bits, see alignment E=2.5e-21 PF07728: AAA_5" amino acids 329 to 450 (122 residues), 22.1 bits, see alignment E=2.6e-08 PF02954: HTH_8" amino acids 575 to 602 (28 residues), 33.5 bits, see alignment (E = 5.6e-12)

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU3747)

Predicted SEED Role

"sigma-54 dependent transcriptional regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JY05 at UniProt or InterPro

Protein Sequence (603 amino acids)

>PFLU_RS18275 sigma-54-dependent Fis family transcriptional regulator (Pseudomonas fluorescens SBW25)
MAEPLAHDTLIQESWRRCRAFGLDHQSTPSFDQLPAEGISQLLESQHSLVQTTHQEVLPY
YENILSNSNCLIMLADNQGQVLTSWGTQRFIEPKLARGFNPGASWRERSSGTNAIGTALA
CAQAVHIEHDEHFLKANRFMTGSAAPIFDAQREIIAVLDVSSDSYLPPSHTLGMVKMMSQ
TVENRLILNLFRGEHFQLTFNTGLSNLDSQWAGLLIFDESGQVLSANRRADNLLGISLSR
VMIDSLFKVSLLELLNQPEGLPFSLQAAGRNRFQCLLRRPKQVPVQARVFTEPTPPKPAS
IGLKTLHFGDVRVEKAVRQAERLLEKDIPLLIHGETGVGKEVFVKALHQASSRSQKAFIA
VNCAAIPAELVESELFGYEKGAFTGANQKGSIGLIRKADKGTLFLDEIGDMPLPTQARLL
RVLQERCVQPVGSSELFPVDLRIISATNRALRELVQAGRFREDLYYRIGGLTLELPPLRE
RTDKRALFQQLWQQHREPTQWAGLSAEVLALFEQHPWPGNLRQVSSVLQVALAMAEEQPI
RPEHLPDDFFVDLNVESPLPASEYLDASIDLNQRLKAVGGNISHLARELGVSRNTLYKRL
RQH