Protein Info for PFLU_RS17470 in Pseudomonas fluorescens SBW25

Annotation: amino acid ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 222 transmembrane" amino acids 20 to 45 (26 residues), see Phobius details amino acids 55 to 78 (24 residues), see Phobius details amino acids 84 to 104 (21 residues), see Phobius details amino acids 138 to 159 (22 residues), see Phobius details amino acids 189 to 209 (21 residues), see Phobius details TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 14 to 110 (97 residues), 103.2 bits, see alignment E=4.4e-34 PF00528: BPD_transp_1" amino acids 37 to 210 (174 residues), 66.8 bits, see alignment E=1.1e-22

Best Hits

Swiss-Prot: 42% identical to YECS_SHIFL: L-cystine transport system permease protein YecS (yecS) from Shigella flexneri

KEGG orthology group: K02029, polar amino acid transport system permease protein (inferred from 100% identity to pfs:PFLU3583)

MetaCyc: 42% identical to cystine ABC transporter membrane subunit (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-290 [EC: 7.4.2.12]; 7.4.2.12 [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]

Predicted SEED Role

"ABC-type amino acid transport system, permease component"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7Y3 at UniProt or InterPro

Protein Sequence (222 amino acids)

>PFLU_RS17470 amino acid ABC transporter permease (Pseudomonas fluorescens SBW25)
MNPAEFLQNAQDFLPILLQGAWVTIQITVLSFLLSSAIGLVLALLKLSPIRALSWTASTI
INVIRGLPIIVQLFYIYFVLPDMGVHLTAFYAGVIGMGIAYSAYQAENFRTGIIAVEQGQ
REAAEALGMRSTLMMRRVILPQAFRIALPPYGNTLVMMLKDSSLVSTITVAEMTRQGQLI
ASSTFQNMTVYTLVALLYLLMSLPLVYGLRRMEQHLGRRKKA