Protein Info for PFLU_RS13635 in Pseudomonas fluorescens SBW25

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 transmembrane" amino acids 25 to 43 (19 residues), see Phobius details amino acids 62 to 81 (20 residues), see Phobius details amino acids 92 to 112 (21 residues), see Phobius details amino acids 118 to 139 (22 residues), see Phobius details amino acids 151 to 174 (24 residues), see Phobius details amino acids 186 to 208 (23 residues), see Phobius details amino acids 253 to 274 (22 residues), see Phobius details amino acids 286 to 307 (22 residues), see Phobius details amino acids 319 to 338 (20 residues), see Phobius details amino acids 344 to 366 (23 residues), see Phobius details amino acids 376 to 398 (23 residues), see Phobius details amino acids 411 to 431 (21 residues), see Phobius details PF07690: MFS_1" amino acids 31 to 393 (363 residues), 158 bits, see alignment E=1.6e-50

Best Hits

Swiss-Prot: 83% identical to NICT_PSEPK: Putative metabolite transport protein NicT (nicT) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2795)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9X3 at UniProt or InterPro

Protein Sequence (436 amino acids)

>PFLU_RS13635 MFS transporter (Pseudomonas fluorescens SBW25)
MPIANAAPATTVADPVNTLYHKITWKLIPFLCFCYLAAYLDRINIGFAKLQMLDQLQFSE
TAFGLGAGLFFVGYILFEVPSNLVLERVGAKIWIARIMITWGLLSACTMLVTSTTQFYIL
RFLLGAAEAGFLPGVLYYLTTWFPTHRRGRIIALFMIGLPLSSVIGGPLSGWIMGHFDHM
GGLRGWQWLFLIEAVPSVLLGVLTFWALPNTYQQAKWLSDEEKALLETELRKDDADGADS
KHGFRDGFFNLKVWMLGGIDFSILLSAYAMGFWMPTFIRNAGVIDTFHIGVLTALPSVAA
LLGMLLIGASSDKHRERRWHIIVPFFIGAAAMATSTFFTHNVAATVALFAIASAAIIGAV
PVFFSLPATFLKGTAAATGFALACSVANIAGLVSNSLMGVAIDVTGSSAGALWFFAGCLI
LSSFLVIALPAKLVNR