Protein Info for PFLU_RS11160 in Pseudomonas fluorescens SBW25

Annotation: xanthine dehydrogenase family protein molybdopterin-binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 743 signal peptide" amino acids 21 to 22 (2 residues), see Phobius details amino acids 42 to 42 (1 residues), see Phobius details transmembrane" amino acids 23 to 41 (19 residues), see Phobius details PF20256: MoCoBD_2" amino acids 33 to 188 (156 residues), 51.2 bits, see alignment E=1.3e-17 amino acids 604 to 685 (82 residues), 58.5 bits, see alignment E=7.9e-20 PF02738: MoCoBD_1" amino acids 334 to 565 (232 residues), 113.7 bits, see alignment E=8e-37

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 100% identity to pfs:PFLU2271)

MetaCyc: 91% identical to 2-deoxy-D-ribose dehydrogenase beta subunit (Pseudomonas simiae)
1.1.2.M2 [EC: 1.1.2.M2]

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.1.2.M2 or 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KAR0 at UniProt or InterPro

Protein Sequence (743 amino acids)

>PFLU_RS11160 xanthine dehydrogenase family protein molybdopterin-binding subunit (Pseudomonas fluorescens SBW25)
MNTRPELLDLLSGEPVNLSRRRFLASTAVGALVIGFGLPLGSARVQAAATVERGTQVPAF
LEIRPDGSVRLLSPFMEGGQGTHTAMAQIVGEELDADPATFVVEAAPPGEAYVVMENGMR
ITGGSMSVRMSYPTMRRLGALARAMLLQAGAEQLGVPLAELTTQPGRVVHAASGRSLGYG
ELAGRALDMPVPDPASITLRDPSQFRWIGKPVKRLDAYDKSTGKALYSIDLKVDGMLHAA
VQHAPRLGMTVGGVRNQAQVEDMKGVHSVHVLPGAVAVVAERWWHAKRAVEAIQADWQEA
APDSTVRVMPADFSSDGFRDFLAAQQGPARDDENEGDVAAALAGAKTTVDATYHNQYLNH
AQLEPPSALARYNADGTLDIWLPNQAPDMFRADIAKRAGLDVAKINLHSPLLGGFFGRHF
LYDSANPYPQAIALAKAVGRPVKVIWSREEEFLRDVLRPVAVVKFRAALDDKGLPVAIEA
VSATEGPTEAIAGKQGEKLDPTALEGLSGKAYAIPNKRIAQIYVKGPAMLGYWRSVGNSL
NDFFYEAFLDELADKGGHDPYELRLHLLRDNPRLTTLLQAAGELSGGWKRGPFTAEDGSR
RARGVAMASPFGSHAAVIAEVSIENGQVKVHDIWQAIDPGSIVNPAIVEAQVNGAVALGL
SQTLLEEAVYVNGLPRARNYDLYPILPPSRMARVHVRVVESGEKMGGIGEPPLPAVAPAV
ANAVAQLTGQRIRSLPMSRHTFS