Protein Info for PFLU_RS10265 in Pseudomonas fluorescens SBW25

Annotation: LysE family translocator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 209 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 42 to 67 (26 residues), see Phobius details amino acids 75 to 92 (18 residues), see Phobius details amino acids 112 to 133 (22 residues), see Phobius details amino acids 145 to 170 (26 residues), see Phobius details amino acids 185 to 204 (20 residues), see Phobius details PF01810: LysE" amino acids 16 to 205 (190 residues), 109.7 bits, see alignment E=6.6e-36

Best Hits

Swiss-Prot: 35% identical to LEUE_CITK8: Leucine efflux protein (leuE) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2084)

Predicted SEED Role

"Threonine efflux protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K5Q2 at UniProt or InterPro

Protein Sequence (209 amino acids)

>PFLU_RS10265 LysE family translocator (Pseudomonas fluorescens SBW25)
MIDLATLAVFSGAVLLLLLSPGPNMAFVISHGVTHGWRGGMASALGIGVADVLLTALTAT
GVTALVASWPPSFDVIRYAGVVYLLWLVFKTLQKSPGSDAAHINRVPLGRVFLQAMLNSL
LNPKALLFFLVFLPQFLRPEAGAIAVQLMVLGGVLTLIAAVFHMLLGIFGGALRRFFAGR
SKGAWLQKWGLATVLTVLAVRLAVMSRPA