Protein Info for PFLU_RS05070 in Pseudomonas fluorescens SBW25

Annotation: TonB-dependent receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 806 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF07660: STN" amino acids 63 to 113 (51 residues), 32.2 bits, see alignment 1.1e-11 PF07715: Plug" amino acids 156 to 253 (98 residues), 73.7 bits, see alignment E=2.4e-24 TIGR01783: TonB-dependent siderophore receptor" amino acids 157 to 806 (650 residues), 374.4 bits, see alignment E=6.2e-116 PF00593: TonB_dep_Rec" amino acids 328 to 774 (447 residues), 238.6 bits, see alignment E=4e-74

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 100% identity to pfs:PFLU1022)

Predicted SEED Role

"Ferrichrome-iron receptor" in subsystem Iron acquisition in Vibrio or Transport of Iron

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K5C7 at UniProt or InterPro

Protein Sequence (806 amino acids)

>PFLU_RS05070 TonB-dependent receptor (Pseudomonas fluorescens SBW25)
MKSTAGGWIKQWLGASALAVSGLALLPLSVAQAQEQQSAQFNFALAAKPLPQALSDFSRV
TGISVIYTDEAPYGLSAPAVSGQMSANQALQRLLSNTGFTFRQIDARTLALEPVPTDGAV
NLGATTISGVGQQAQDSTSYQPPPTSSVMRSGGLLLETPQTVNVVPAQVLRDQTPRNLDD
ALGNISGITQANTLASTQDAVMLRGFGDNRNGSIMRDGMPIVQGRALNATAERVEVLKGP
ASLLYGIQDPGGVVNIVSKKPELVQSTALTVRGSTFGDGKNGSGGSLDTTGPIGDSGLAY
RLIVDHEDEDYWRNFGTHRESLIAPSLAWYGDTTKLLFAYEHREFLTPFDRGTAIDKSNH
PLDIPATRRLDEPFNNMEGRSDLYRFEADHDLNDDWKAHFGYSWNRETYDASQVRVSAVN
DNGTLTRRMDGTQGAITTDRFTTASLEGKVKLLGMQHDLVFGLDDEYRKIYRADLIRQGS
RGTFNYNNPVYGNEVAGTTVSAVDSAQTDLLRSDSLFFQDAIHLNDQWIFVAGARYQMYD
QYAGKGVPFKANTNGNGQAWVPRAGLVYRYTDELSFYGSYTESFKPNSTIAALNDGSTLT
GDLTPEESKSWELGAKLDIPGRITASAALFTIDKRNVLVSVGSGANTIYSIAGQVRSRGL
ELDATGQLTDKWSVIGSYAYTDAEDIKDNDPTLEGNRLQNVAKHTGSLSVAYDFGNIFGG
DQLRVGTGARYVGERAGDAANDFNLPGYTVADAFATYDTKIDGQKVKFQLNVKNMFDRVY
YTSAASRLFVSMGDARQVSVSSTLEF