Protein Info for PFLU_RS04030 in Pseudomonas fluorescens SBW25
Annotation: flavodoxin
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00380, sulfite reductase (NADPH) flavoprotein alpha-component [EC: 1.8.1.2] (inferred from 100% identity to pfs:PFLU0808)Predicted SEED Role
"Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB"
MetaCyc Pathways
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
- assimilatory sulfate reduction I (4/4 steps found)
- assimilatory sulfate reduction III (3/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.8.1.2
Use Curated BLAST to search for 1.8.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3K4M7 at UniProt or InterPro
Protein Sequence (841 amino acids)
>PFLU_RS04030 flavodoxin (Pseudomonas fluorescens SBW25) MLKKTLFQLHWFFGITAGLVLALMGISGAAVSFQDEMLQALNPSVLTVEKRDAGVLPPAE LVRKLEATEGQTVAMLFVESESGKAARVFFTPPPGERRGQLRYFDPYTGDYMGDVVGQDV FGFLLQFHRFLVLGDTGRNITGACTLILVFFCLSGLYLRWPRQAANWRAWLTLDWRKKGR AFNWDLHSVFGTWCLLAYLFSALTGLYWSYDWYSQGLTQLLSDAPQHERVRKRGPAPEGA APVANYAAMWSSIYSNAGPGLSAYNIRMPAVAGQPATVFYLLKDSPHDRALNQINLDPAT GEVKSHDRYASKSLGSRLLTSVYALHTGSYFGLAGRIVLTVSSLLMPLFFITGWLLYLDR RRKKRQVRDARKGLSANHSDAPAWLIGFASQSGFAEQLAWQTAGQLQAAGLPVKVQPLGS VSQDDLRQSENALFVVSTFGDGEAPDSARGFERSVLGQDLSLSGLNYSVLALGDRQYEHF CGFARRLHFWLTNQGGNPLFAPVEVDSGDTTALLHWQQQLGQLTGHAPAAAWPTAQYENW TLSQRTLLNPDSSGSQVYLLGLTSPAPHTWQAGDLVEVLPRNGPWAIEHFLEGLGLAGSD AVVFEGLAQSLNQALATRQLPDNRAHLVGLHAQALVNALVPLGMREYSIASIASEGVLEL IVRQERHPDGSLGLASGWLTEHAAIGSSISLRLRRNSGFHLPEMPVPLILLGNGTGLAGL RSLLKARIADGQQRNWLLFGERNIAHDYLCQDELQGWLASGDLALLDLAFSRDQEEKIYV QDRLRESAEVLRKWLAEGAAIYVCGSLQGMAAGVDQALVDILGNEAVERLIEQGRYRRDV Y