Protein Info for OKFHMN_24470 in Escherichia coli ECRC100

Name: gndA
Annotation: NADP-dependent phosphogluconate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00873: 6-phosphogluconate dehydrogenase (decarboxylating)
  • amino acids 5 to 467 (463 residues), 771.4 bits, E=1.6e-236
• PF03446: NAD_binding_2
  • amino acids 5 to 174 (170 residues), 170.7 bits, E=2.6e-54
• PF00393: 6PGD
  • amino acids 179 to 466 (288 residues), 452.4 bits, E=7.4e-140

Best Hits

Swiss-Prot: 100% identical to 6PGD_ECOLI: 6-phosphogluconate dehydrogenase, decarboxylating (gnd) from Escherichia coli (strain K12)

KEGG orthology group: K00033, 6-phosphogluconate dehydrogenase [EC: 1.1.1.44] (inferred from 100% identity to eco:b2029)

MetaCyc: 100% identical to 6-phosphogluconate dehydrogenase, decarboxylating (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydrogenase (decarboxylating). [EC: 1.1.1.44]

Predicted SEED Role

"6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)" in subsystem Pentose phosphate pathway (EC 1.1.1.44)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (17/17 steps found)
• pentose phosphate pathway (8/8 steps found)
• pentose phosphate pathway (oxidative branch) I (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Glutathione metabolism
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.44

Protein Sequence (468 amino acids)

>OKFHMN_24470 NADP-dependent phosphogluconate dehydrogenase (Escherichia coli ECRC100)
MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAENPGKKLVPYYTVKEF
VESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGF
NFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAAVAEDGEPCVTYIGADGAGH
YVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQIFTEWNNGELSSYLIDITKDIFT
KKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLITESVFARYISSLKDQRVAAS
KVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYAQGFSQLRAASEEYNWDLNYGEIAKIF
RAGCIIRAQFLQKITDAYAENPQIANLLLAPYFKQIADDYQQALRDVVAYAVQNGIPVPT
FAAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDKEGVFHTEWLD