Protein Info for OKFHMN_24325 in Escherichia coli ECRC100

Name: wcaC
Annotation: colanic acid biosynthesis glycosyltransferase WcaC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 TIGR04015: colanic acid biosynthesis glycosyltransferase WcaC" amino acids 1 to 405 (405 residues), 897.9 bits, see alignment E=4.2e-275 PF13439: Glyco_transf_4" amino acids 13 to 223 (211 residues), 38.5 bits, see alignment E=2.4e-13 PF20706: GT4-conflict" amino acids 284 to 360 (77 residues), 27 bits, see alignment E=4.4e-10 PF13692: Glyco_trans_1_4" amino acids 286 to 353 (68 residues), 32.2 bits, see alignment E=2.4e-11 PF00534: Glycos_transf_1" amino acids 289 to 348 (60 residues), 30.3 bits, see alignment E=5.7e-11

Best Hits

Swiss-Prot: 100% identical to WCAC_ECOLI: Putative colanic acid biosynthesis glycosyl transferase WcaC (wcaC) from Escherichia coli (strain K12)

KEGG orthology group: K13684, putative colanic acid biosynthesis glycosyltransferase [EC: 2.4.-.-] (inferred from 100% identity to eco:b2057)

MetaCyc: 100% identical to colanic acid biosynthesis galactosyltransferase WcaC (Escherichia coli K-12 substr. MG1655)
2.4.1.-

Predicted SEED Role

"Colanic acid biosynthesis glycosyl transferase WcaC" in subsystem Colanic acid biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.-.-

Use Curated BLAST to search for 2.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (405 amino acids)

>OKFHMN_24325 colanic acid biosynthesis glycosyltransferase WcaC (Escherichia coli ECRC100)
MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGKESVSHQNYPQVIKHTP
RMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHVLHSYWLNLKSVVRFCEKV
KNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCPTLNNYPPVKIDRAHQLVAGK
RQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIINNGIDMATEAILADLPPVRETQG
KPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHTFGKFSPFTAGNVVNHGFETDKRKL
MSALNQMDALVFSSRVDNYPLILCEALSIGVPVIATHSDAAREVLQKSGGKTVSEEEVLQ
LVQLSKPEIAQALFGTTLAEFSQRSRAAYSGQQMLEEYVNFYQNL