Protein Info for OKFHMN_21875 in Escherichia coli ECRC100

Name: hyfF
Annotation: hydrogenase 4 subunit F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 521 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 30 to 49 (20 residues), see Phobius details amino acids 69 to 90 (22 residues), see Phobius details amino acids 110 to 125 (16 residues), see Phobius details amino acids 131 to 151 (21 residues), see Phobius details amino acids 163 to 185 (23 residues), see Phobius details amino acids 207 to 230 (24 residues), see Phobius details amino acids 249 to 271 (23 residues), see Phobius details amino acids 283 to 301 (19 residues), see Phobius details amino acids 317 to 338 (22 residues), see Phobius details amino acids 372 to 393 (22 residues), see Phobius details amino acids 413 to 432 (20 residues), see Phobius details amino acids 452 to 472 (21 residues), see Phobius details PF00662: Proton_antipo_N" amino acids 63 to 96 (34 residues), 25.5 bits, see alignment (E = 1e-09) PF00361: Proton_antipo_M" amino acids 127 to 420 (294 residues), 189.3 bits, see alignment E=9.6e-60

Best Hits

Swiss-Prot: 98% identical to HYFF_ECOLI: Hydrogenase-4 component F (hyfF) from Escherichia coli (strain K12)

KEGG orthology group: K12141, hydrogenase-4 component F [EC: 1.-.-.-] (inferred from 100% identity to etw:ECSP_3425)

Predicted SEED Role

"Hydrogenase-4 component F"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (521 amino acids)

>OKFHMN_21875 hydrogenase 4 subunit F (Escherichia coli ECRC100)
MFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGITLLLILALWVVQTAADAGEIFA
AGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFT
MLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGLFGTVL
VYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDA
HSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFIL
VQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKY
GTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLL
LLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIR
ILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER