Protein Info for OKFHMN_20910 in Escherichia coli ECRC100

Name: gabT
Annotation: 4-aminobutyrate--2-oxoglutarate transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00700: 4-aminobutyrate transaminase
  • amino acids 9 to 421 (413 residues), 540 bits, E=1.6e-166
• PF00202: Aminotran_3
  • amino acids 16 to 420 (405 residues), 500.9 bits, E=1.2e-154

Best Hits

Swiss-Prot: 99% identical to GABT_ECOLI: 4-aminobutyrate aminotransferase GabT (gabT) from Escherichia coli (strain K12)

KEGG orthology group: K07250, 4-aminobutyrate aminotransferase / (S)-3-amino-2-methylpropionate transaminase [EC: 2.6.1.19 2.6.1.22] (inferred from 99% identity to eco:b2662)

MetaCyc: 99% identical to 4-aminobutyrate aminotransferase GabT (Escherichia coli K-12 substr. MG1655)
5-aminovalerate transaminase. [EC: 2.6.1.48]; 4-aminobutyrate transaminase. [EC: 2.6.1.48, 2.6.1.19]; Acetylornithine transaminase. [EC: 2.6.1.48, 2.6.1.19, 2.6.1.11]

Predicted SEED Role

"Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)" (EC 2.6.1.19)

MetaCyc Pathways

• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• superpathway of L-arginine and L-ornithine degradation (13/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (11/11 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-lysine degradation I (7/7 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-ornithine biosynthesis I (5/5 steps found)
• superpathway of 4-aminobutanoate degradation (3/3 steps found)
• L-lysine degradation X (5/6 steps found)
• 4-aminobutanoate degradation I (2/2 steps found)
• 4-aminobutanoate degradation II (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• GABA shunt I (3/4 steps found)
• GABA shunt II (3/4 steps found)
• L-lysine degradation IV (2/5 steps found)
• L-valine degradation I (4/8 steps found)
• L-lysine degradation III (2/6 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)
• nicotine degradation I (pyridine pathway) (6/17 steps found)
• superpathway of L-lysine degradation (14/43 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Butanoate metabolism
• Glutamate metabolism
• Lysine biosynthesis
• Lysine degradation
• Propanoate metabolism
• Urea cycle and metabolism of amino groups
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.11, 2.6.1.19

Use Curated BLAST to search for 2.6.1.11 or 2.6.1.19 or 2.6.1.22 or 2.6.1.48

Protein Sequence (426 amino acids)

>OKFHMN_20910 4-aminobutyrate--2-oxoglutarate transaminase (Escherichia coli ECRC100)
MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYLDFAGGIAVLNTGHLHP
KVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFAKKTLLVTTGSEAVENAVK
IARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMGLMPGHVYRALYPCPLHGISE
DDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAATPAFMQRLRALCDEHGIMLIADE
VQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPLAGVTGRAEVMDAVAPGGLGGTYAG
NPIACVAALEVLKVFEQENLLQKANDLGQKLKDGLLAIAEKHPEIGDVRGLGAMIAIELF
EDGDHNKPDAKLTAEIVARARDKGLILLSCGPYYNVLRILVPLTIEDAQIRQGLEIISQC
FAEAKQ