Protein Info for OKFHMN_18895 in Escherichia coli ECRC100

Name: ygiS
Annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00496: SBP_bac_5" amino acids 75 to 453 (379 residues), 283.1 bits, see alignment E=1.9e-88

Best Hits

Swiss-Prot: 99% identical to YGIS_ECOLI: Probable deoxycholate-binding periplasmic protein YgiS (ygiS) from Escherichia coli (strain K12)

KEGG orthology group: K02035, peptide/nickel transport system substrate-binding protein (inferred from 99% identity to eco:b3020)

MetaCyc: 42% identical to murein tripeptide ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-268 [EC: 7.4.2.6]

Predicted SEED Role

"Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)" in subsystem ABC transporter oligopeptide (TC 3.A.1.5.1) or Sex pheromones in Enterococcus faecalis and other Firmicutes (TC 3.A.1.5.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (535 amino acids)

>OKFHMN_18895 ABC transporter substrate-binding protein (Escherichia coli ECRC100)
VYTRNLLWLVSLVSAAPLYAADVPTNTQLAPQQVFRYNNHSDPGTLDPQKVEENTAAQIV
LDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQWSDGQPLTAEDFVLGWQR
AVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKATDDRTLEVTLEQPVPWFTTML
AWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWVVNEKITARKNPKYRDAQHTVLQ
QVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIEKSLPGEIRIIPRLNSEYYNFNLEK
PPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATTLTPPEVKGFSATTFDELQKPMSERVA
MAKALLKQAGYDASHPLRFELFYNKYDLHEKTAIALSSEWKKWLGAQVTLRTMEWKTYLD
ARRAGDFMLSRQSWDATYNDASSFLNTLKSDSEENVGHWKNAQYDALLNQAMQITDATKR
NALYQQAEVIVNQQAPLIPIYYQPLIKLLKPYVGGFPLHNPQDYVYSKELYIKAH