Protein Info for OKFHMN_10330 in Escherichia coli ECRC100

Name: hrpB
Annotation: ATP-dependent helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 809 TIGR01970: ATP-dependent helicase HrpB" amino acids 4 to 808 (805 residues), 1274.5 bits, see alignment E=0 PF00270: DEAD" amino acids 21 to 164 (144 residues), 45.8 bits, see alignment E=9e-16 PF00271: Helicase_C" amino acids 208 to 327 (120 residues), 37.8 bits, see alignment E=3.1e-13 PF08482: HrpB_C" amino acids 664 to 796 (133 residues), 180.8 bits, see alignment E=2.4e-57

Best Hits

Swiss-Prot: 98% identical to HRPB_ECOLI: ATP-dependent RNA helicase HrpB (hrpB) from Escherichia coli (strain K12)

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 100% identity to ece:Z0159)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (809 amino acids)

>OKFHMN_10330 ATP-dependent helicase HrpB (Escherichia coli ECRC100)
VSSLPVAAVLPELLTALDYAPQVLLSAPTGAGKSTWLPLQLLAHPGINGKIILLEPRRLA
ARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEGVLTRMIQRDPELSGVGLV
ILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLDNDRLQQMLPEAPVVISEGRS
FPVERRYLPLPTHQRFDDAVAVATAEMLRQESGSLLLFLPGVGEIQRVQEQLASRIGSDV
LLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAETSLTIEGIRLVVDCAQERVARFDP
RTGLTRLITQRISQASMTQRAGRAGRLEPGICLHLIAKEQAERATAQSEPEILQSDLSGL
LMELLQWGCSDPAQMSWLDQPPTVNLLAAKRLLQMLGALDGERLSAQGQKMAALGNDPRL
AAMLVSAKSDDEAATAAKIAAILEEPPRMGNSDLGVAFSRNQPAWQQRSQQLLKRLNVRG
GEADSSLIAPLLAGAFADRIARRRGQDGRYQLANGMGAMLDADDALSRHEWLIAPLLLQG
SASPDARILLALPVDIDELVQRCPQLVQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPL
AKPSEDELHQAMLNGIRDKGLSVLNWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLAT
LETWLLPHMAGVHSLRGLKSLDIYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYH
EDNPPALAVRMQEMFGEATNPTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQ
KEMKGRYPKHVWPDDPANTAPTRRTKKYS