Protein Info for OHPLBJKB_02421 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Tryptophan biosynthesis protein TrpCF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00218: IGPS
  • amino acids 5 to 251 (247 residues), 337.7 bits, E=3.6e-105
• PF00697: PRAI
  • amino acids 257 to 447 (191 residues), 222.7 bits, E=3.5e-70

Best Hits

Swiss-Prot: 100% identical to TRPC_ECOLI: Tryptophan biosynthesis protein TrpCF (trpC) from Escherichia coli (strain K12)

KEGG orthology group: K13498, indole-3-glycerol phosphate synthase / phosphoribosylanthranilate isomerase [EC: 4.1.1.48 5.3.1.24] (inferred from 100% identity to eco:b1262)

MetaCyc: 100% identical to fused indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylanthranilate isomerase. [EC: 5.3.1.24]; Indole-3-glycerol-phosphate synthase. [EC: 5.3.1.24, 4.1.1.48]

Predicted SEED Role

"Indole-3-glycerol phosphate synthase (EC 4.1.1.48) / Phosphoribosylanthranilate isomerase (EC 5.3.1.24)" in subsystem Tryptophan synthesis or Auxin biosynthesis (EC 4.1.1.48, EC 5.3.1.24)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• L-tryptophan biosynthesis (6/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.48 or 5.3.1.24

Protein Sequence (452 amino acids)

>OHPLBJKB_02421 Tryptophan biosynthesis protein TrpCF (Escherichia coli HS(pFamp)R (ATCC 700891))
MQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDALQGARTAFILECKKASPS
KGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPIVSQIAPQPILCKDFIIDP
YQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGVLTEVSNEEEQERAIALGAKV
VGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGINTYAQVRELSHFANGFLIGSALM
AHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGAIYGGLIFVATSPRCVNVEQAQEVM
AAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLHGNEEQLYIDTLREALPAHVAIWKALS
VGETLPAREFQHVDKYVLDNGQGGSGQRFDWSLLNGQSLGNVLLAGGLGADNCVEAAQTG
CAGLDFNSAVESQPGIKDARLLASVFQTLRAY