Protein Info for OHPLBJKB_02262 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Vitamin B12 transporter BtuB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF07715: Plug" amino acids 41 to 151 (111 residues), 79 bits, see alignment E=5.8e-26 TIGR01783: TonB-dependent siderophore receptor" amino acids 42 to 695 (654 residues), 225.2 bits, see alignment E=8.7e-71 PF00593: TonB_dep_Rec" amino acids 230 to 662 (433 residues), 192.2 bits, see alignment E=4.6e-60 PF14905: OMP_b-brl_3" amino acids 396 to 589 (194 residues), 36.1 bits, see alignment E=6.1e-13

Best Hits

Swiss-Prot: 99% identical to YNCD_ECOLI: Probable TonB-dependent receptor YncD (yncD) from Escherichia coli (strain K12)

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 99% identity to eco:b1451)

MetaCyc: 99% identical to pyrroloquinoline quinone outer membrane transporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-629

Predicted SEED Role

"Probable tonB-dependent receptor yncD precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (700 amino acids)

>OHPLBJKB_02262 Vitamin B12 transporter BtuB (Escherichia coli HS(pFamp)R (ATCC 700891))
MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTPAAVSVVDGEEMRLATP
RINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIRGIRLYVDGIPATMPDGQG
QTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQQPPTIEASSYYGSFGSWRYG
LKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQKNLANAKLGVRIDDASKLSLIFN
SVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTIKQTQAGLRYERSLSSRDDMSVMMY
AGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQGIDSRWTHRGELGVPVTFTTGLNYEN
MSENRKGYNNFRLNSGMPEYGQKGELRRDERNLMWNIDPYLQTQWQLSEKLSLDAGVRYS
SVWFDSNDHYITPGNGDDSGDASYHKWLPAGSLKYAMTDAWNIYLAAGRGFETPTINELS
YRADGQSGMNFGLKPSTNDTIEIGSKTRIGDGLLSLALFQTDTDDEIVVDSSSGGRTTYK
NAGKTRRQGAELAWDQRFAGDFRVNASWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFA
SIGYVPEDGWYAGTEARYMGDIMADDENTAKAPSYTLIGLFTGYKYNYHNLTVDLFGRVD
NLFDKEYVGSVIVNESNGRYYEPAPGRNYGVGVNIAWRFE