Protein Info for OHPLBJKB_01547 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Putative pseudouridine transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 transmembrane" amino acids 29 to 59 (31 residues), see Phobius details amino acids 107 to 128 (22 residues), see Phobius details amino acids 135 to 154 (20 residues), see Phobius details amino acids 192 to 217 (26 residues), see Phobius details amino acids 220 to 244 (25 residues), see Phobius details amino acids 261 to 270 (10 residues), see Phobius details amino acids 287 to 312 (26 residues), see Phobius details amino acids 325 to 348 (24 residues), see Phobius details TIGR00804: nucleoside transporter, NupC family" amino acids 1 to 346 (346 residues), 443.4 bits, see alignment E=4.3e-137 PF07670: Gate" amino acids 32 to 130 (99 residues), 56.8 bits, see alignment E=2.8e-19 PF07662: Nucleos_tra2_C" amino acids 135 to 344 (210 residues), 242.4 bits, see alignment E=4.1e-76

Best Hits

Swiss-Prot: 100% identical to PSUT_ECOLI: Putative pseudouridine transporter (psuT) from Escherichia coli (strain K12)

KEGG orthology group: K03317, concentrative nucleoside transporter, CNT family (inferred from 100% identity to eco:b2164)

MetaCyc: 90% identical to putative nucleoside transporter (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Inner membrane transport protein YeiM, in cluster with pseudouridine metabolism operon"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (350 amino acids)

>OHPLBJKB_01547 Putative pseudouridine transporter (Escherichia coli HS(pFamp)R (ATCC 700891))
MSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVLPAIIFVTALISLLYYIGVMGLLIR
ILGSIFQKALNISKIESFVAVTTIFLGQNEIPAIVKPFIDRMNRNELFTAICSGMASIAG
SMMIDYAGMGVPIDYLLAASLMAIPGGILFARILSPATEPSQVTFENLSFSETPPKSFIE
AAASGAMTGLKIAAGVATVVMAFVAIIALINGIIGGIGGWFGFANASLESIFGYVLAPLA
WIMGVDWSDANLAGSLIGQKLAINEFVAYLSFSPYLQTGGTLEVKTIAIISFALCGFANF
GSIGVVVGAFSAISPKRAPEIAQLGLRALAAATLSNLMSATIAGFFIGLA