Protein Info for OH686_19035 in Pseudomonas sp. S08-1

Annotation: Inner membrane protein YihY, formerly thought to be RNase BN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 transmembrane" amino acids 27 to 50 (24 residues), see Phobius details amino acids 93 to 112 (20 residues), see Phobius details amino acids 133 to 157 (25 residues), see Phobius details amino acids 173 to 194 (22 residues), see Phobius details amino acids 204 to 228 (25 residues), see Phobius details amino acids 235 to 264 (30 residues), see Phobius details amino acids 276 to 290 (15 residues), see Phobius details TIGR00765: YihY family inner membrane protein" amino acids 11 to 267 (257 residues), 242.1 bits, see alignment E=4.3e-76 PF03631: Virul_fac_BrkB" amino acids 22 to 266 (245 residues), 199.5 bits, see alignment E=3.9e-63

Best Hits

Swiss-Prot: 72% identical to Y4558_PSEA7: UPF0761 membrane protein PSPA7_4558 (PSPA7_4558) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K07058, membrane protein (inferred from 73% identity to pfv:Psefu_1511)

Predicted SEED Role

"Ribonuclease BN (EC 3.1.-.-)" in subsystem LMPTP YfkJ cluster or tRNA processing (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (405 amino acids)

>OH686_19035 Inner membrane protein YihY, formerly thought to be RNase BN (Pseudomonas sp. S08-1)
MRQRLDDVVEFWRFLLQRFLADRAINSAAALTYTTLFAVVPVMTVTFATLSAVPAFQDTG
EQIQSFIFHNFVPSSGETVQQYLREFTTQARHLTWVGVALLAVTAFFMLVTIEKTFNAIW
RVRQPRRGISSFLLYWAILSLGPLLLGAGFAVTTYIASLSLLSGPDALIGAKTLLGFAPL
LFSTAAFTLIYSAVPNARVPLAHALTGGLFAAVLVEIAKGIFGLFVSLFPGYQLIYGAFA
TVPLFLLWVYLSWLIVLFGAELVCNLSSSRIWRRRAVPRLLVLLGVLRVFHDRQQRGRPV
RHLDVQREGWLLPEDEWEEVLDFLEREQLICRTSGGGWVLCRDLEHYSLHRLLGHSPWPL
PRPGQLPEQLDEVWYPALRQALAQLHEQQAALFDGSLAQWLRAKE