Protein Info for OH686_17515 in Pseudomonas sp. S08-1

Annotation: 23S rRNA (adenine(2503)-C(2))-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00048: 23S rRNA (adenine(2503)-C(2))-methyltransferase
  • amino acids 5 to 358 (354 residues), 471.5 bits, E=7.7e-146
• PF21016: RlmN_N
  • amino acids 7 to 66 (60 residues), 100.7 bits, E=3e-33
• PF04055: Radical_SAM
  • amino acids 110 to 284 (175 residues), 59.1 bits, E=6.4e-20

Best Hits

Swiss-Prot: 90% identical to RLMN_PSEMY: Dual-specificity RNA methyltransferase RlmN (rlmN) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K06941, ribosomal RNA large subunit methyltransferase N [EC: 2.1.1.-] (inferred from 90% identity to pmy:Pmen_3503)

MetaCyc: 59% identical to 23S rRNA m2A2503 methyltransferase/tRNA m2A37 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11586 [EC: 2.1.1.192]; 2.1.1.- [EC: 2.1.1.192]

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase (EC 2.1.1.-)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.192

Protein Sequence (381 amino acids)

>OH686_17515 23S rRNA (adenine(2503)-C(2))-methyltransferase (Pseudomonas sp. S08-1)
MSDVAKTNLLGLTQPEMESFFESIGEKRFRAGQVMKWIHHFGVEDFDAMSNLGKALREKL
SACAEIRGPEIVSQDISSDGTRKWVVRVASGSCVETVYIPQGGRGTLCVSSQAGCALDCS
FCSTGKQGFNSDLTAAEIIGQVWIANKSFGTVPAKIDRAITNVVMMGMGEPLLNFDNVVP
AMQIMMDDLGYGISKRKVTLSTSGVVPMIDKLATVTDVSLALSLHAPNDELRNQLVPLNK
KYPLEVLLASCRRYIEGLGEKRFLTVEYTLLKDVNDQPEHAAQMIALLKDFPCKINLIPF
NPFPHSGYERPSNNAIRRFQDLLHKAGFNVTVRTTRGDDIDAACGQLVGQVQDRTRRSER
YIAVRQLGNEAAEPRDAANRN