Protein Info for OH686_16320 in Pseudomonas sp. S08-1

Annotation: murein biosynthesis integral membrane protein MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 514 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 31 to 48 (18 residues), see Phobius details amino acids 83 to 111 (29 residues), see Phobius details amino acids 133 to 153 (21 residues), see Phobius details amino acids 161 to 180 (20 residues), see Phobius details amino acids 186 to 205 (20 residues), see Phobius details amino acids 237 to 266 (30 residues), see Phobius details amino acids 274 to 292 (19 residues), see Phobius details amino acids 313 to 334 (22 residues), see Phobius details amino acids 354 to 374 (21 residues), see Phobius details amino acids 384 to 404 (21 residues), see Phobius details amino acids 410 to 429 (20 residues), see Phobius details amino acids 450 to 468 (19 residues), see Phobius details amino acids 480 to 502 (23 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 2 to 504 (503 residues), 525.5 bits, see alignment E=7.2e-162 PF03023: MurJ" amino acids 28 to 480 (453 residues), 496 bits, see alignment E=9.4e-153 PF14667: Polysacc_synt_C" amino acids 353 to 502 (150 residues), 35.7 bits, see alignment E=9.2e-13

Best Hits

Swiss-Prot: 70% identical to MURJ_SALTY: Probable lipid II flippase MurJ (murJ) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03980, virulence factor (inferred from 85% identity to pmk:MDS_1039)

MetaCyc: 69% identical to lipid II flippase MurJ (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-286

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (514 amino acids)

>OH686_16320 murein biosynthesis integral membrane protein MurJ (Pseudomonas sp. S08-1)
MNLLKSLAAVSSLTMVSRVLGFVRDTLIARIFGAGVASDAFVVAFKLPNLLRRIFAEGAF
SQAFVPILAEYKTQQGEEATRTFLAYIAGLLSLVLALVTLLGILAAPWIVWISAPGFADE
PERFALTVDLLRVTFPYILLISLSSLVGAVLNTWNRFSVPAFVPTLLNVSMIVFTVWLAP
YFDPPIMALGWAVLVGGLAQFLWQLPALKKTGMLVLPRLSLRDTGVWRVLKQMGPAIFGV
SVSQISLIINTIFASFLVAGSVSWMYYADRLMELPSGVLGVALGTILLPALAKTYASADR
EEYSKLLDWGLRLCFLLVLPCTLALAIIAEPLIVSLFQYGKFTAHDSAMTQQALVAYSVG
LLALILVKILAPGFYAQQNIKTPVRIAIVSLLATQAMNALFVFGLDLRHAGLALAISLAA
CLNAGLLYWQLRSKRMYQPQPGWPKFLAKLVLAVLAMTAVLLLVMHHMPAWGEGGMLMRL
LRLGALVAAGTVTYFGMLLLLGFRPRDFARRAIH