Protein Info for OH686_16185 in Pseudomonas sp. S08-1

Annotation: DNA repair protein RadA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 TIGR00416: DNA repair protein RadA" amino acids 1 to 450 (450 residues), 665.1 bits, see alignment E=2.5e-204 PF18073: Rubredoxin_2" amino acids 8 to 35 (28 residues), 38.9 bits, see alignment (E = 1.6e-13) PF06745: ATPase" amino acids 79 to 146 (68 residues), 38.5 bits, see alignment E=2.6e-13 PF13481: AAA_25" amino acids 81 to 221 (141 residues), 45.6 bits, see alignment E=1.9e-15 PF09848: SLFN-g3_helicase" amino acids 92 to 215 (124 residues), 20.4 bits, see alignment E=8.4e-08 PF13541: ChlI" amino acids 362 to 429 (68 residues), 33.1 bits, see alignment E=1.4e-11 PF05362: Lon_C" amino acids 371 to 453 (83 residues), 28.9 bits, see alignment E=2.5e-10

Best Hits

Swiss-Prot: 91% identical to RADA_PSEAE: DNA repair protein RadA (radA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K04485, DNA repair protein RadA/Sms (inferred from 94% identity to pmy:Pmen_0756)

Predicted SEED Role

"DNA repair protein RadA" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (455 amino acids)

>OH686_16185 DNA repair protein RadA (Pseudomonas sp. S08-1)
MAKAKRMYGCTECGATFPKWAGQCGECGAWNTLNETMIESGGAAAPSGRGGWAGQQAQIK
TLAEVSVEEVPRFSTVSGELDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRLP
ALYVTGEESQQQVAMRARRLELPQDKLKVMTETCIESIIDVARREQPKVMVIDSIQTIFT
EQLQSAPGGVAQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVTNPSAIFLTRAQEAVPGSVVMATW
EGTRPMLVEVQALVDTSHMANPKRVTLGLDQNRLAMLLAVLHRHGGIPTYDQDVFLNVVG
GVKVLETASDLALMAAVISSLRNKPLPHDLLVFGEIGLSGEIRPVPSGQERLKEAAKHGF
KRAIVPKGNAPKEAPAGLQVIAVTRLEQALDALFE