Protein Info for OH686_12660 in Pseudomonas sp. S08-1

Annotation: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 630 TIGR00136: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA" amino acids 7 to 620 (614 residues), 922.2 bits, see alignment E=7e-282 PF01134: GIDA" amino acids 8 to 399 (392 residues), 583.1 bits, see alignment E=9.2e-179 PF12831: FAD_oxidored" amino acids 8 to 142 (135 residues), 37.9 bits, see alignment E=4.3e-13 PF00890: FAD_binding_2" amino acids 8 to 43 (36 residues), 21.1 bits, see alignment 4.8e-08 PF21680: GIDA_C_1st" amino acids 460 to 554 (95 residues), 89.6 bits, see alignment E=5.4e-29 PF13932: GIDA_C" amino acids 558 to 614 (57 residues), 82.1 bits, see alignment 6e-27

Best Hits

Swiss-Prot: 93% identical to MNMG_PSEMY: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K03495, glucose inhibited division protein A (inferred from 93% identity to pmy:Pmen_4618)

MetaCyc: 70% identical to 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (630 amino acids)

>OH686_12660 tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (Pseudomonas sp. S08-1)
MDFPSRFDVIVIGGGHAGTEAALAAARMGVKTLLLTHNVETLGQMSCNPAIGGIGKSHLV
KEIDALGGAMALATDKGGIQFRVLNSRKGPAVRATRAQADRVLYKAAIREILENQPNLWI
FQQAADDLIVEQDQVKGVVTQMGLRFMADNVVLTTGTFLGGLIHIGLQNYSGGRAGDPPS
IALAKRLRELPLRVGRLKTGTPPRIDGRSVDFSVMTEQPGDTPIPVMSFLGNKDMHPRQI
SCWMTHTNARTHEIIASNLDRSPMYSGVIEGIGPRYCPSIEDKIHRFADKDSHQVFIEPE
GLTTHELYPNGISTSLPFDVQLEIVRSIRGMENAHIVRPGYAIEYDYFDPRDLKYSLETK
VIGGLFFAGQINGTTGYEEAGAQGLLAGANAALRAQGKDAWCPRRDEAYIGVLVDDLITL
GTQEPYRMFTSRAEYRLILREDNADLRLTEKGRELGLVDDTRWAAFEAKREGIVQEEQRL
KSTWVRPGTPQGDAIVARFGTPLAHEYNLLNLLARPEIDYAALAEVTEAPQVDAQVAEQV
EIKTKYAGYIDRQQEEIERLRASEDTRLPADIDYAGISGLSKEIQHKLGNARPETLGQAG
RIPGVTPAAISLLLIHLKKRGAGRQLEQSA