Protein Info for OH686_03550 in Pseudomonas sp. S08-1

Annotation: Na+ or Li+/H+ antiporter NhaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 transmembrane" amino acids 20 to 46 (27 residues), see Phobius details amino acids 58 to 78 (21 residues), see Phobius details amino acids 89 to 107 (19 residues), see Phobius details amino acids 119 to 138 (20 residues), see Phobius details amino acids 144 to 162 (19 residues), see Phobius details amino acids 195 to 217 (23 residues), see Phobius details amino acids 237 to 257 (21 residues), see Phobius details amino acids 294 to 312 (19 residues), see Phobius details amino acids 318 to 335 (18 residues), see Phobius details amino acids 347 to 365 (19 residues), see Phobius details amino acids 390 to 409 (20 residues), see Phobius details amino acids 449 to 464 (16 residues), see Phobius details amino acids 472 to 491 (20 residues), see Phobius details PF06450: NhaB" amino acids 3 to 492 (490 residues), 732.9 bits, see alignment E=2.5e-224 PF03600: CitMHS" amino acids 66 to 324 (259 residues), 64 bits, see alignment E=1.4e-21

Best Hits

Swiss-Prot: 81% identical to NHAB_PSEPF: Na(+)/H(+) antiporter NhaB (nhaB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03314, Na+:H+ antiporter, NhaB family (inferred from 81% identity to pfo:Pfl01_2589)

Predicted SEED Role

"Na+/H+ antiporter NhaB" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (494 amino acids)

>OH686_03550 Na+ or Li+/H+ antiporter NhaB (Pseudomonas sp. S08-1)
MAGALAHNFLGSSPSWYKLTILFFLLANPLVLWAAGPVAAGWLLVGEFIFTLGMALKCYP
LLPGGLLVLQAILLGLATPKALYEELAHNFPVILLLMFMVAGIYFMKDLLLFLFSRLLLG
VRSKALLALLFCLLSAFLSAFLDALTVTAVIIAATLGFYAVYHRVASGRGAQDEEFLEAE
AVELHREDLEQFRAFLRSLLMHGAVGTALGGVCTLVGEPQNLLIGHEMGWNFADFFLRVA
PVSIPVLAAGLATCVLLERLGWFGYGAQLPASVRQVLAEHANASDAQRTPEQRAALLVQG
LAAVLLIIGLALHVAEVGLIGLSVIVLITAFSGITDEHRIGRAFQDALPFTALLVTFFAV
VAVIQQQQLFTPLIQAVLALPAEQQPGMLFIANGLLSAISDNVFVATIYITEVKQAFLAG
NMTREHFEALAVAVNTGTNLPSVATPNGQAAFLFLLTSSIAPLIRLSYGRMVWMALPYTI
VMGGLGWWAVSYWL