Protein Info for NOLOHH_20680 in Escherichia coli ECOR27

Name: treA
Annotation: alpha,alpha-trehalase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF01204: Trehalase" amino acids 55 to 532 (478 residues), 600.2 bits, see alignment E=6e-184 PF22422: MGH1-like_GH" amino acids 159 to 512 (354 residues), 78.4 bits, see alignment E=1.2e-25 PF06202: GDE_C" amino acids 170 to 441 (272 residues), 34 bits, see alignment E=2.4e-12

Best Hits

Swiss-Prot: 100% identical to TREA_ECOSE: Periplasmic trehalase (treA) from Escherichia coli (strain SE11)

KEGG orthology group: K01194, alpha,alpha-trehalase [EC: 3.2.1.28] (inferred from 100% identity to ebw:BWG_1022)

MetaCyc: 100% identical to periplasmic trehalase (Escherichia coli K-12 substr. MG1655)
Alpha,alpha-trehalase. [EC: 3.2.1.28]

Predicted SEED Role

"Trehalase (EC 3.2.1.28)" in subsystem Trehalose Uptake and Utilization (EC 3.2.1.28)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.28

Use Curated BLAST to search for 3.2.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (565 amino acids)

>NOLOHH_20680 alpha,alpha-trehalase (Escherichia coli ECOR27)
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQ
KTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHID
GLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWDSYFTMLGLAESGHWDKVADM
VANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELLAQHEGDAALKQYLPQMQKEYAY
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIY
RDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAM
ANQYETLANARQKGIEKYMWNDQQGWYADYDLKSHKVRNQLTAAALFPLYVNAAAKDRAN
KMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCD
NVPATRPTVKSATTQPSTKEAQPTP