Protein Info for NOLOHH_06595 in Escherichia coli ECOR27

Name: hemC
Annotation: hydroxymethylbilane synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01379: Porphobil_deam
  • amino acids 13 to 220 (208 residues), 292.3 bits, E=1.9e-91
• TIGR00212: hydroxymethylbilane synthase
  • amino acids 13 to 304 (292 residues), 374.5 bits, E=1.5e-116
• PF03900: Porphobil_deamC
  • amino acids 233 to 303 (71 residues), 90 bits, E=1e-29

Best Hits

Swiss-Prot: 100% identical to HEM3_ECOSE: Porphobilinogen deaminase (hemC) from Escherichia coli (strain SE11)

KEGG orthology group: K01749, hydroxymethylbilane synthase [EC: 2.5.1.61] (inferred from 99% identity to eco:b3805)

MetaCyc: 99% identical to hydroxymethylbilane synthase (Escherichia coli K-12 substr. MG1655)
Hydroxymethylbilane synthase. [EC: 2.5.1.61]

Predicted SEED Role

"Porphobilinogen deaminase (EC 2.5.1.61)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 2.5.1.61)

MetaCyc Pathways

• superpathway of heme b biosynthesis from glutamate (10/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
• superpathway of heme b biosynthesis from glycine (7/8 steps found)
• dipyrromethane cofactor biosynthesis (3/4 steps found)
• tetrapyrrole biosynthesis II (from glycine) (3/4 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (17/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (16/36 steps found)
• superpathway of bacteriochlorophyll a biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.61

Protein Sequence (320 amino acids)

>NOLOHH_06595 hydroxymethylbilane synthase (Escherichia coli ECOR27)
MIMTVTSMLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTRGDVILDTPL
AKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICEREDPRDAFVSNNY
DSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLDNGEYDAIILAVAG
LKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAALNHHETALRVTAERA
MNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGERRGAPQNAEQMGISLAEE
LLNNGAREILAEVYNGDAPA