Protein Info for NOLOHH_00300 in Escherichia coli ECOR27

Annotation: conjugal transfer protein TrbI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 transmembrane" amino acids 81 to 102 (22 residues), see Phobius details PF03743: TrbI" amino acids 235 to 416 (182 residues), 127.9 bits, see alignment E=2.1e-41

Best Hits

Predicted SEED Role

"Inner membrane protein forms channel for type IV secretion of T-DNA complex (VirB10)" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (427 amino acids)

>NOLOHH_00300 conjugal transfer protein TrbI (Escherichia coli ECOR27)
MENKTPDEIEKEMMEHEQLHDDEINENINKESTRKIKTVAEIEQEIREKEAAKLAEESHD
VTEKHQPEVTKLKKSAKDNFLKKILFVAVAVIIVAIAGLKIYQKYKAPPKEETDKTIKTK
EATMNKRNNLGQEVDPFRTEEPEQPTVEDRGNDNPFASSGTGTTPQTAPVAFSRALAVTA
EGGQAGSNTRTRKEEKAFGTVSSESSLTQTKDEAGNDSESEVTAVKRIPYNPDLYIPENT
PISCALTRRFVSDVAGKLQCVLTDDVYSASKKTRLLDKGSTASLVYKTGMLNHGQARVFV
MATKIRTQQPPFMDIPLVDTDAAGELGEAGVDGWIDTHFKDRFLGAAMLGMIPDIANAAT
DNVASTDRNTDYTQNSREAFAELAKTAFENSVNIPPTIYKNQGEIITLITGRDIDFSKVY
TLKYRGK