Protein Info for NIAGMN_20250 in Escherichia coli ECRC102

Name: cscB
Annotation: Sucrose permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 415 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details amino acids 49 to 68 (20 residues), see Phobius details amino acids 79 to 98 (20 residues), see Phobius details amino acids 107 to 128 (22 residues), see Phobius details amino acids 148 to 167 (20 residues), see Phobius details amino acids 173 to 190 (18 residues), see Phobius details amino acids 219 to 236 (18 residues), see Phobius details amino acids 259 to 281 (23 residues), see Phobius details amino acids 289 to 312 (24 residues), see Phobius details amino acids 318 to 336 (19 residues), see Phobius details amino acids 346 to 367 (22 residues), see Phobius details amino acids 376 to 398 (23 residues), see Phobius details PF01306: LacY_symp" amino acids 5 to 410 (406 residues), 642.7 bits, see alignment E=4.8e-197 TIGR00882: oligosaccharide:H+ symporter" amino acids 9 to 399 (391 residues), 593.4 bits, see alignment E=1e-182 PF03825: Nuc_H_symport" amino acids 19 to 397 (379 residues), 27.1 bits, see alignment E=4.2e-10 PF07690: MFS_1" amino acids 19 to 323 (305 residues), 71 bits, see alignment E=1.8e-23 PF12832: MFS_1_like" amino acids 20 to 381 (362 residues), 77.4 bits, see alignment E=2.3e-25

Best Hits

Swiss-Prot: 100% identical to CSCB_ECOLX: Sucrose permease (cscB) from Escherichia coli

KEGG orthology group: K02532, MFS transporter, OHS family, lactose permease (inferred from 99% identity to ecp:ECP_2386)

Predicted SEED Role

"Sucrose permease, major facilitator superfamily" in subsystem Sucrose utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (415 amino acids)

>NIAGMN_20250 Sucrose permease (Escherichia coli ECRC102)
MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLTGTELGTLYSVNQFTSI
LFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLLQSNFSVGLILGALFFGLG
YLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFFAGIFFSISPHINFWLVSLFG
AVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRNFWVFVIFIVGTWSFYNIFDQQL
FPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAIIPFFVNRVGPKNALLIGVVIMALR
ILSCALFVNPWIISLVKLLHAIEVPLCVISVFKYSVANFDKRLSSTIFLIGFQIASSLGI
VLLSTPTGILFDHAGYQTVFFAISGIVCLMLLFGIFFLSKKREQIVMETPVPSAI