Protein Info for NIAGMN_19620 in Escherichia coli ECRC102

Name: hyfR
Annotation: DNA-binding transcriptional regulator HyfR

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 670 PF13185: GAF_2" amino acids 168 to 311 (144 residues), 42.5 bits, see alignment E=2.6e-14 PF01590: GAF" amino acids 169 to 311 (143 residues), 51.1 bits, see alignment E=7.2e-17 PF00158: Sigma54_activat" amino acids 347 to 513 (167 residues), 250.6 bits, see alignment E=2.2e-78 PF14532: Sigma54_activ_2" amino acids 350 to 518 (169 residues), 76.6 bits, see alignment E=8.3e-25 PF07728: AAA_5" amino acids 371 to 489 (119 residues), 29.8 bits, see alignment E=2e-10

Best Hits

Swiss-Prot: 99% identical to HYFR_ECOLI: DNA-binding transcriptional activator HyfR (hyfR) from Escherichia coli (strain K12)

KEGG orthology group: K12146, hydrogenase-4 transcriptional activator (inferred from 100% identity to ecs:ECs3353)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (670 amino acids)

>NIAGMN_19620 DNA-binding transcriptional regulator HyfR (Escherichia coli ECRC102)
MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPLPPPFSPVQLIELHVLK
SNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAELRFIRTHGSRFTPQDCTLF
NWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDITNAVLSHLDLDDLIADVARE
IHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACLPRNMPGDSVLLTQTLQTRQPTL
THRADDLFLWQRDPLLHLLASNGCESSLLIPLTFGNHTPGALLLAHTSTTLFSEENCQLL
QHIADRIAIAVGNADAWRSMTDLQESLQQENHQLSEQLLSNLGVGDIIYQSQAMEDLLQQ
VDIVAKSDSTVLICGETGTGKEVIARAIHQLSPRRDKPLVKINCAAIPASLLESELFGHD
KGAFTGAINTHRGRFEIADGGTLFLDEIGDLPLELQPKLLRVLQEREIERLGGSRTIPVN
VRVIAATNRDLWQMVEDRQFRSDLFYRLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHM
NRAIDAIPTEALRQLMSWDWPGNVRELENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNE
DSALRSSMAQLLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQ
RLGISVREVL