Protein Info for NIAGMN_17815 in Escherichia coli ECRC102

Name: ptrA
Annotation: pitrilysin

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 962 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00675: Peptidase_M16" amino acids 54 to 191 (138 residues), 133.8 bits, see alignment E=7.4e-43 PF05193: Peptidase_M16_C" amino acids 216 to 394 (179 residues), 91.9 bits, see alignment E=6.9e-30 amino acids 681 to 857 (177 residues), 71.6 bits, see alignment E=1.2e-23 PF16187: Peptidase_M16_M" amino acids 400 to 675 (276 residues), 193.1 bits, see alignment E=8.7e-61

Best Hits

Swiss-Prot: 100% identical to PTRA_ECO57: Protease 3 (ptrA) from Escherichia coli O157:H7

KEGG orthology group: K01407, protease III [EC: 3.4.24.55] (inferred from 100% identity to eck:EC55989_3097)

MetaCyc: 100% identical to protease 3 (Escherichia coli K-12 substr. MG1655)
Pitrilysin. [EC: 3.4.24.55]

Predicted SEED Role

"Protease III precursor (EC 3.4.24.55)" (EC 3.4.24.55)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.24.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (962 amino acids)

>NIAGMN_17815 pitrilysin (Escherichia coli ECRC102)
MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQYQAIRLDNGMVVLLVSD
PQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYPQADSLAEYLKMHGGSHNA
STAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAERERNAVNAELTMARTRDGMR
MAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQALKDFHEKYYSANLMKAVIYSNKP
LPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKGIIIHYVPALPRKVLRVEFRIDNNS
AKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVEGISANSDPIVNGNSGVLAISASLTDK
GLANRDQVVAAIFSYLNLLREKGIDKQYFDELANVLDIDFRYPSITRDMDYVEWLADTMI
RVPVEHTLDAVNIADRYDAKAVKERLAMMTPQNARIWYISPKEPHNKTAYFVDAPYQVDK
ISEQTFADWQQKAANIALSLPELNPYIPDDFSLIKSEKKYDHPELIVDESNLRVVYAPSR
YFSSEPKADVSLILRNPKAMDSARNQVMFALNDYLAGLALDQLSNQASVGGISFSTNANN
GLMVNANGYTQRLPQLFQALLEGYFSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMP
AQMLSQVPYFSRDERRKILPSITLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDV
QKQLGADGSEWCRNKDVVVDKKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLG
QIVQPWFYNQLRTEEQLGYAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAE
AKLRAMKPDEFAQIQQAVITQMLQAPQTLGEEALKLSKDFDRGNMRFDSRDKIVAQIKLL
TPQKLADFFHQAVVEPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEK
NE