Protein Info for MPMX26_02950 in Acinetobacter radioresistens SK82

Annotation: Glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 525 PF04262: Glu_cys_ligase" amino acids 18 to 388 (371 residues), 496.4 bits, see alignment E=2e-153 TIGR01434: glutamate--cysteine ligase" amino acids 19 to 523 (505 residues), 540.4 bits, see alignment E=2.1e-166

Best Hits

Swiss-Prot: 49% identical to GSH1_PSEMY: Glutamate--cysteine ligase (gshA) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 74% identity to aby:ABAYE0116)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (525 amino acids)

>MPMX26_02950 Glutamate--cysteine ligase (Acinetobacter radioresistens SK82)
MSQPESISQVLIPTWVDSSLFKGMLRGVEREGLRMQSNGFISQGPHPHSLGAALTHPHIT
TDYSEALMEFITAPQPDIQAMLAELTDIHALVYQHLEHQEKLWPLSMPCMLDREEDSIQL
AQYGSSNIGRFKTLYRRGLGVRYGRRMQTIAGVHYNLSFPDELFEQLQQHEQDKALTQLS
LQDYRSHRYFGLIRNFIRLTPLVMYLVGASPSVCQCFLTGREHHLLPLVKGTLYLPYATA
LRMGRFGYQNSAQKQLGIHYNNLSGYLDGLQKAVKTPYAQFSRLGLKDADGEPLQINDHV
LQIENEYYSLVRPKQVPRDGETPSEALANRGVGYVELRAVDINPYNPIGIDENTAGFLEV
LALYCLLKDSPDLLPEEQDQIENNQAEVVNRGRAPNSHITERGQEQSLEQWAQAHVVAMQ
PLAVLLDEGYHTQLYSHALQLMQQRIQQVDETLSAHVIADTIKWGGTWNLGSHLAQEHAN
FYEQHALTPEKQEYFTKLAQTSLEQQQQLEADTRISFEDYLAHYQ