Protein Info for MPMX26_02833 in Acinetobacter radioresistens SK82

Annotation: O-acetyl-L-homoserine sulfhydrylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 5 to 423 (419 residues), 669.5 bits, E=8.5e-206
• PF01053: Cys_Met_Meta_PP
  • amino acids 6 to 422 (417 residues), 473.4 bits, E=7.8e-146
• PF01041: DegT_DnrJ_EryC1
  • amino acids 58 to 185 (128 residues), 31.6 bits, E=2.2e-11
• PF00155: Aminotran_1_2
  • amino acids 62 to 183 (122 residues), 43.1 bits, E=6.6e-15
• PF00266: Aminotran_5
  • amino acids 88 to 212 (125 residues), 39.2 bits, E=9.1e-14

Best Hits

Swiss-Prot: 54% identical to METY_LEPME: O-acetyl-L-homoserine sulfhydrylase (metY) from Leptospira meyeri

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 92% identity to abb:ABBFA_000290)

MetaCyc: 54% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (6/10 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
• homocysteine and cysteine interconversion (1/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Protein Sequence (425 amino acids)

>MPMX26_02833 O-acetyl-L-homoserine sulfhydrylase (Acinetobacter radioresistens SK82)
MTYKDETLAIHAGYSPEATTKAVAVPIYQTTSYSFDNTQHGADLFDLKVQGNIYTRIMNP
TTAVLEQRIAALEGGIGALALASGMAAITYAIQTIAEAGDNIASVSTLYGGTYNLFAHTL
PKQGIEVRFFDYQDPEALHSLIDNKTKLVFVESIGNPLGNIIDLEAISKIAHEYGVPVVV
DNTVATPVLLKPFEFGADIVVHSLTKYIGGHGNSIGGAIVDSGKFPWGKYPDRFKVLNTP
DPSYHGVNYVEALGEAAYIARARVVPLRNTGAAISPMNAFLILQGLETLSLRMECHTENA
LKIAEYLKAHPKVKWVNYAGLTDHPQHALAQKYVQGKPSAILSFGVQDGLAGGTRFIDAL
QLFTRLVNIGDAKSLACHPATTTHRQLNEQELKSAGVSEDMVRLSIGIEHADDLIADLEQ
ALAAV