Protein Info for MPMX26_02159 in Acinetobacter radioresistens SK82

Annotation: Elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 11 to 190 (180 residues), 182.2 bits, E=2e-57
• TIGR01393: elongation factor 4
  • amino acids 11 to 604 (594 residues), 952.9 bits, E=6.2e-291
• TIGR00231: small GTP-binding protein domain
  • amino acids 14 to 184 (171 residues), 65.8 bits, E=4.1e-22
• PF03144: GTP_EFTU_D2
  • amino acids 214 to 284 (71 residues), 40.1 bits, E=1e-13
• PF00679: EFG_C
  • amino acids 408 to 493 (86 residues), 72.8 bits, E=4.9e-24
• PF06421: LepA_C
  • amino acids 496 to 602 (107 residues), 168.9 bits, E=7.6e-54

Best Hits

Swiss-Prot: 96% identical to LEPA_ACIBY: Elongation factor 4 (lepA) from Acinetobacter baumannii (strain AYE)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 96% identity to aby:ABAYE0938)

MetaCyc: 73% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (605 amino acids)

>MPMX26_02159 Elongation factor 4 (Acinetobacter radioresistens SK82)
MAQAKKSVDIKNIRNFSIIAHIDHGKSTLADRFIQMCGGLQDREMQAQVLDSMELERERG
ITIKAASVTLYYTHPNGQEYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQ
SVANCYTAIEQGLEVLPILNKIDLPQAEPERVIHEIEEIIGIEATHAPTCSAKTGMGVDK
VLETLVEVIPAPEGDRDAPLQALIVDSWFDNYLGVVSLVRIKQGRVRKGDKMLVKSTGQT
HIITSVGIFNPKHTETGMLEAGEVGFIIAGIKDIFGAPVGDTITLASTPEVGTLPGFKKV
KPQVYAGLFPIDASDFEPFREALHKLQINDSALFFEPESSDALGFGFRCGFLGMLHMEIV
QERLEREYDLDLITSAPTVVYEALTKAGDTIYIDSPSKMPDGSTVEDLREPIAECHILVP
QEYLGNVMTLCIERRGVQKDMKFLGNQVSITFEIPMAEVVMDFFDRLKSCSRGFASLDYN
FVRFESSSLVKVDVLINGEKVDALAMICHRNDARHRGIALVEKMKDLIPRQMFDVAIQAA
IGAQIIARSTVKAMRKNVLAKCYGGDVSRKKKLLSKQKEGKKRMKQVGSVEIPQEAFLAV
LKVER