Protein Info for MPMX26_01687 in Acinetobacter radioresistens SK82

Annotation: 2,4-dienoyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 674 PF00724: Oxidored_FMN" amino acids 7 to 332 (326 residues), 304.5 bits, see alignment E=4.4e-94 PF07992: Pyr_redox_2" amino acids 377 to 640 (264 residues), 94.8 bits, see alignment E=2.6e-30 PF00890: FAD_binding_2" amino acids 378 to 417 (40 residues), 20.8 bits, see alignment 8.1e-08 PF13450: NAD_binding_8" amino acids 380 to 418 (39 residues), 33.3 bits, see alignment 1.9e-11

Best Hits

Swiss-Prot: 62% identical to FADH_ECOLI: 2,4-dienoyl-CoA reductase (fadH) from Escherichia coli (strain K12)

KEGG orthology group: K00219, 2,4-dienoyl-CoA reductase (NADPH2) [EC: 1.3.1.34] (inferred from 84% identity to aby:ABAYE1661)

MetaCyc: 62% identical to 2,4-dienoyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
2,4-dienoyl-CoA reductase (NADPH). [EC: 1.3.1.34]

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.34

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (674 amino acids)

>MPMX26_01687 2,4-dienoyl-CoA reductase (Acinetobacter radioresistens SK82)
MSYYPHLLAPLDLGFTTLKNRVLMGSMHVGLEEAPQGYERMAAFYAERARGEVGLIVTGG
IAPNDAGVTFAGGSKLDSIAEAEKHKIITQAVHDAGGKIAMQILHTGRYSYQADPVAPSA
IQAPINPSKPKELSSAEVWQTVSDFVNCAQMAQYAGYDGVEIMGSEGYLINEFIAARTNH
RDDEWGGSYEQRIRFPIEIVKRTREAVGENFIIIYRLSMLDLVEGGSTLDEVVYLAKEIE
KAGATIINTGIGWHEARIPTIATKVPRAAFTWVTEKLKGEVKLPLITSNRINTPEMAEYV
LSSGQADMISMARPMLADAEFVLKAREGRSDEINTCIACNQACLDHIFSMKIASCLVNPR
ACHETELIFKESGSPKQIAVIGAGPAGLSFAIYAASRGHQVKIFESSNQIGGQFNIAKTI
PGKEEFYETIRYFQRQIELQPNIQLHLNHLATYEELSQANFDDIVIATGVTPRHLEIEGA
NHPKALSYLEVLKERLPVGKRVAIIGAGGIGFDTAEYLTHEGESASLNPEKFYDEWGIDT
TYHHTGGLKQPKIEPSLREVYLLQRKQKAVGANLGKTTGWIHRTGLKHKHVQMMSGVNYE
KIDDQGLHITVDGKPMLLEVDNVVICAGQESYTAMFEQLQADGKRVHLIGGAREAGELDA
KRAIRQGAELAAII