Protein Info for MPMX26_01294 in Acinetobacter radioresistens SK82

Annotation: Ribosomal protein S12 methylthiotransferase RimO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 PF00919: UPF0004" amino acids 5 to 93 (89 residues), 73.6 bits, see alignment E=1.7e-24 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 5 to 438 (434 residues), 349.1 bits, see alignment E=3.5e-108 TIGR01125: ribosomal protein S12 methylthiotransferase RimO" amino acids 5 to 438 (434 residues), 603.7 bits, see alignment E=2.3e-185 PF04055: Radical_SAM" amino acids 142 to 320 (179 residues), 84.9 bits, see alignment E=1.2e-27 PF18693: TRAM_2" amino acids 376 to 442 (67 residues), 72.5 bits, see alignment E=3.8e-24

Best Hits

Swiss-Prot: 95% identical to RIMO_ACIBC: Ribosomal protein S12 methylthiotransferase RimO (rimO) from Acinetobacter baumannii (strain ACICU)

KEGG orthology group: K14441, ribosomal protein S12 methylthiotransferase [EC: 2.-.-.-] (inferred from 95% identity to abn:AB57_2321)

MetaCyc: 69% identical to ribosomal protein S12 methylthiotransferase RimO (Escherichia coli K-12 substr. MG1655)
RXN0-6366 [EC: 2.8.4.4]

Predicted SEED Role

"Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.-.-.-

Use Curated BLAST to search for 2.-.-.- or 2.8.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (447 amino acids)

>MPMX26_01294 Ribosomal protein S12 methylthiotransferase RimO (Acinetobacter radioresistens SK82)
MKSPKVGFVSLGCPKALVDSERILTQLKTEGYQVASDYDGADLVVVNTCGFIESAVQESL
DAIGEAMSENGRVIVTGCLGKDEDKIRQMHPNVLKVTGAAAYEDVMSAVHQYVPAPPKHN
PFIDLVPEQGVRLTPKHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPVGSVLEEAAALKR
AGVREILVISQDTSAYGVDTKYKLDFWNGQPVKTKFFDMCEALGQLGIWVRLHYVYPYPH
VDAVIDLMAQGKILPYLDIPFQHASPRILKLMKRPAHSENTLERLKVWREKCPDLVIRST
FVVGFPGETEEDFQILLEWLKEAQLDRVGCFTYSPVEGATANDLPDHVPEEVKQERYERF
MQVQQEISAAKLQKRIGQTMTVLVDNLEEEFPVAVARSYADAPEIDGNVFVEDIDKSQIK
SGDLLEVEITDADEYDLFAKLIRIKSV