Protein Info for MPMX26_00767 in Acinetobacter radioresistens SK82

Annotation: Carbamoyl-phosphate synthase small chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01368: carbamoyl-phosphate synthase, small subunit
  • amino acids 5 to 375 (371 residues), 465.2 bits, E=6.9e-144
• PF00988: CPSase_sm_chain
  • amino acids 7 to 135 (129 residues), 173.7 bits, E=1.4e-55
• PF00117: GATase
  • amino acids 195 to 372 (178 residues), 178.6 bits, E=1.1e-56

Best Hits

Swiss-Prot: 92% identical to CARA_ACIAD: Carbamoyl-phosphate synthase small chain (carA) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K01956, carbamoyl-phosphate synthase small subunit [EC: 6.3.5.5] (inferred from 92% identity to aci:ACIAD2860)

MetaCyc: 69% identical to carbamoyl-phosphate synthetase small subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase small chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• superpathway of arginine and polyamine biosynthesis (8/17 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (379 amino acids)

>MPMX26_00767 Carbamoyl-phosphate synthase small chain (Acinetobacter radioresistens SK82)
MSIPAILALADGTIFKGKSIGASGCTTGEVVFNTAMTGYQEILTDPSYAQQLVTLTYPHI
GNTGCNTEDAESGRIQKVWANGLIIRDLPLLHSNFRADMSLGEYLEQNNVVAIADIDTRK
LTRILRDKGAQNGCILAGENITAEEALEKARAFGGLEGLDLAKECCDPEGFEWTEGSWAL
GQGFTQPELKYHVVAYDYGVKTNILRMLADRGCKLTVVPAQTPVEKVLALNPDGVFLSNG
PGDPAACGYAIEAVKTIVETTTLPVFGICLGHQILALASGAATLKMGHGHHGANHPVQNI
EDGTVMITSQNHGFAVDESTLPEVLRVTHRSLFDGTNQGIHRTDKPAFSFQGHPEASPGP
HDCAPLFDHFIELIEASKK