Protein Info for MPMX26_00766 in Acinetobacter radioresistens SK82

Annotation: Carbamoyl-phosphate synthase large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1059 (1058 residues), 1575.8 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 250.3 bits, E=5e-78
  • amino acids 673 to 874 (202 residues), 126 bits, E=5.3e-40
• PF02787: CPSase_L_D3
  • amino acids 426 to 505 (80 residues), 89 bits, 5.7e-29
• PF02655: ATP-grasp_3
  • amino acids 673 to 845 (173 residues), 32.3 bits, E=3.7e-11
• PF02222: ATP-grasp
  • amino acids 682 to 845 (164 residues), 36.7 bits, E=1.1e-12
• PF07478: Dala_Dala_lig_C
  • amino acids 699 to 843 (145 residues), 29.8 bits, E=1.5e-10
• PF02142: MGS
  • amino acids 962 to 1043 (82 residues), 82.7 bits, 5.9e-27

Best Hits

Swiss-Prot: 69% identical to CARB_BUCAI: Carbamoyl-phosphate synthase large chain (carB) from Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 91% identity to abb:ABBFA_000786)

MetaCyc: 75% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• superpathway of arginine and polyamine biosynthesis (8/17 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1076 amino acids)

>MPMX26_00766 Carbamoyl-phosphate synthase large chain (Acinetobacter radioresistens SK82)
MPKRTDIKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPAM
ADATYIEPITWQTVAAIIEKERPDAVLPTMGGQTALNCALALDANGVLEKYNVELIGATK
EAIEKAEDRKLFDQAMRKIGLECPKAAIAESMEEALEIQARFGFPVIIRPSFTMGGSGGG
IAYNREEFLEICERGFDLSPTKQLLIDESLIGWKEYEMEVVRDKNDNCIIVCAIENFDPM
GVHTGDSITVAPAQTLTDKEYQLMRNASLAVLREIGVETGGSNVQFGINPKDGRMVIIEM
NPRVSRSSALASKATGFPIAKIAAKLAVGYTLDELKNDITGGTTPASFEPAIDYVVTKVP
RFNFEKFPQADATLTTQMKSVGEVMAIGRNFQESVNKALRGLEVGASGFDEKIEVGTEGA
REKILQELKVPGPERIWYVADAFRHGFTLDEVFAATNIDRWFLVQIEDIINTENQIKTLG
FGDLNADNIRSFKRKGLSDLRIANLMGISQKQFRKHRWNLGVTPVYKRVDTCAAEFESDT
AYMYSTYDEECESNPSNRDKIMVIGGGPNRIGQGIEFDYCCVHAALAMREDGYETIMVNC
NPETVSTDYDTSDRLYFEPITLEDVLEIVRTEKPKGIIVQYGGQTPLKLARALEEAGAPI
IGTSPDAIDRAEDRERFQQMIQRLQLRQPNNSIVKSAEEGMAEAAKVGYPLVVRPSYVLG
GRAMEIVYNDEELKRYLRDAVQASNEAPVLLDRFLDDAIEVDVDCVSDGKDVVIGGIMQH
IEQAGIHSGDSACSIPPYSLSKEVQDEMRRQTIAMAKELGVIGLMNVQFAVKGDDVYILE
VNPRASRTVPFVSKCIGESLAKVAARCMAGQSLESQGFTAEIIPEHFSVKEAVFPFNKFP
GVDPILGPEMKSTGEVMGVGKTFGEAFYKAVLGANERLPGLPTEGEVKHAFISVRDSDKP
RAVGIAKQLIDLGFKILATDGTFKVISEAGLECERVNKVTEGRPNIVDRIKNGEIHLVIN
TTEGKKAQEDSFSIRRSALQGKVYNTTTLNGADAVCQALAIKLPMDVYRLQDLTKG