Protein Info for MPMX19_06859 in Azospirillum sp. SherDot2
Annotation: Acetophenone carboxylase delta subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01474, N-methylhydantoinase B [EC: 3.5.2.14] (inferred from 95% identity to azl:AZL_c03120)Predicted SEED Role
"N-methylhydantoinase B (EC 3.5.2.14)" in subsystem Hydantoin metabolism (EC 3.5.2.14)
MetaCyc Pathways
- creatinine degradation II (2/5 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.2.14
Use Curated BLAST to search for 3.5.2.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (662 amino acids)
>MPMX19_06859 Acetophenone carboxylase delta subunit (Azospirillum sp. SherDot2) MFDRMKLQVLANHARSAAENMAHTLQRTAHSAFVKETEDFTVMLLNRRGETFGVPMELGA TWYPGLTYGRAIDMIEDYRPGDVAFTNDPYSGFVATHAPDTHLWKPVFYEDEIIAWTGGH IHNTDMGGAVPASLSRALTEIHQEGIRFPPMKLVREGVFDEQILRIMTTNVRKPDLNIGD IKALVGALNTGERKILAMVEKFGKTAFLDGVDALLDHAEAQARDILRAMPDGRWEFADYA DEDSVTANPCRLKLTLTIRGDEAVLDFTGSDPQLASSLNVPSGGDPRHTILLVGVYYVLY TLDPKILLNTGLTRPFTCITPEGTVLNPTFPAAVGMRSLTCARLRSVIFGAFSQAVPERL PAAPAGNNCIVNVMTTDERTGSRIIAAVNPVVGGGGGMPHRDGTNGSGADAAYLKNTPIE ITEMETPVEFVRYGLAADSGGAGRWRGGLATHMAFRVFSPDTRITARNRDRGFFRPWGVL GGRAAGLSDMVVNPGTPGERRLGNIDTAVLQPGDVLDIRSAGGGGRGDPFTRESWRVAQD VARGYVSVEAAEREYGVVIRDGVLNTQATDRLRADRTAPTGHFHFGPEREGYERQWTPDA YEFLTRTLAGLPIHWRFFAKTEIFSRMAGRDGAGGVEAALAEVYARFPEMPPSKALVREA AE