Protein Info for MPMX19_06850 in Azospirillum sp. SherDot2

Annotation: putative tartrate dehydrogenase/decarboxylase TtuC'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 TIGR02089: tartrate dehydrogenase" amino acids 1 to 346 (346 residues), 557.7 bits, see alignment E=4.6e-172 PF00180: Iso_dh" amino acids 5 to 327 (323 residues), 298.5 bits, see alignment E=3.9e-93

Best Hits

Swiss-Prot: 52% identical to TTUC4_AGRVI: Probable tartrate dehydrogenase/decarboxylase TtuC' (ttuC') from Agrobacterium vitis

KEGG orthology group: K07246, tartrate dehydrogenase/decarboxylase / D-malate dehydrogenase [EC: 1.1.1.83 1.1.1.93 4.1.1.73] (inferred from 95% identity to azl:AZL_c03210)

MetaCyc: 54% identical to D-malate/3-isopropylmalate dehydrogenase (decarboxylating) (Escherichia coli K-12 substr. MG1655)
Tartrate dehydrogenase. [EC: 1.1.1.93, 4.1.1.73]; 3-isopropylmalate dehydrogenase. [EC: 1.1.1.93, 4.1.1.73, 1.1.1.85]; D-malate dehydrogenase (decarboxylating). [EC: 1.1.1.93, 4.1.1.73, 1.1.1.85, 1.1.1.83]

Predicted SEED Role

"Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83)" (EC 1.1.1.83, EC 1.1.1.93, EC 4.1.1.73)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.85

Use Curated BLAST to search for 1.1.1.83 or 1.1.1.85 or 1.1.1.93 or 4.1.1.73

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (347 amino acids)

>MPMX19_06850 putative tartrate dehydrogenase/decarboxylase TtuC' (Azospirillum sp. SherDot2)
MKTHNIAIIKGDGIGHPVVDAAWEVLQAVAAQSGFALTGTELPWSCAHYKETGVMMPADG
IDSLRKYDAILLGAVGWPAEVPDAVSLHGLLLPIRKAFDQYANVRPHRLLPGVEGPLRRG
GFDILCIRENTEGEYAGAGGRIHQGTPQEVAVETGVFTRMGVERILRFAFAQARARRGKL
ASVTKSNAQKYSMVFWDEITRQLAAEHPDVEVTSYHVDAMAARMVTAPESLDVVVASNLF
GDILTDLGAAIQGGLGFAASANLNPDRKAPSMFEPVHGSAPDIAHLGIANPIAAIWSGAM
MLEHLGEKAAADRVMAALGDTTGRGIGTVPGKDRTDAITAAVLSALS