Protein Info for MPMX19_06850 in Azospirillum sp. SherDot2

Annotation: putative tartrate dehydrogenase/decarboxylase TtuC'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02089: tartrate dehydrogenase
  • amino acids 1 to 346 (346 residues), 557.7 bits, E=4.6e-172
• PF00180: Iso_dh
  • amino acids 5 to 327 (323 residues), 298.5 bits, E=3.9e-93

Best Hits

Swiss-Prot: 52% identical to TTUC4_AGRVI: Probable tartrate dehydrogenase/decarboxylase TtuC' (ttuC') from Agrobacterium vitis

KEGG orthology group: K07246, tartrate dehydrogenase/decarboxylase / D-malate dehydrogenase [EC: 1.1.1.83 1.1.1.93 4.1.1.73] (inferred from 95% identity to azl:AZL_c03210)

MetaCyc: 54% identical to D-malate/3-isopropylmalate dehydrogenase (decarboxylating) (Escherichia coli K-12 substr. MG1655)
Tartrate dehydrogenase. [EC: 1.1.1.93, 4.1.1.73]; 3-isopropylmalate dehydrogenase. [EC: 1.1.1.93, 4.1.1.73, 1.1.1.85]; D-malate dehydrogenase (decarboxylating). [EC: 1.1.1.93, 4.1.1.73, 1.1.1.85, 1.1.1.83]

Predicted SEED Role

"Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83)" (EC 1.1.1.83, EC 1.1.1.93, EC 4.1.1.73)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• D-malate degradation (1/1 steps found)
• methylgallate degradation (3/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (4/8 steps found)
• gentisate degradation II (1/4 steps found)
• superpathway of vanillin and vanillate degradation (5/10 steps found)
• butanol and isobutanol biosynthesis (engineered) (3/8 steps found)
• syringate degradation (4/12 steps found)
• aliphatic glucosinolate biosynthesis, side chain elongation cycle (10/30 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• Glyoxylate and dicarboxylate metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.85

Use Curated BLAST to search for 1.1.1.83 or 1.1.1.85 or 1.1.1.93 or 4.1.1.73

Protein Sequence (347 amino acids)

>MPMX19_06850 putative tartrate dehydrogenase/decarboxylase TtuC' (Azospirillum sp. SherDot2)
MKTHNIAIIKGDGIGHPVVDAAWEVLQAVAAQSGFALTGTELPWSCAHYKETGVMMPADG
IDSLRKYDAILLGAVGWPAEVPDAVSLHGLLLPIRKAFDQYANVRPHRLLPGVEGPLRRG
GFDILCIRENTEGEYAGAGGRIHQGTPQEVAVETGVFTRMGVERILRFAFAQARARRGKL
ASVTKSNAQKYSMVFWDEITRQLAAEHPDVEVTSYHVDAMAARMVTAPESLDVVVASNLF
GDILTDLGAAIQGGLGFAASANLNPDRKAPSMFEPVHGSAPDIAHLGIANPIAAIWSGAM
MLEHLGEKAAADRVMAALGDTTGRGIGTVPGKDRTDAITAAVLSALS