Protein Info for MPMX19_06654 in Azospirillum sp. SherDot2

Annotation: Putative asparagine synthetase [glutamine-hydrolyzing]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 565 (564 residues), 451.5 bits, E=2.5e-139
• PF13522: GATase_6
  • amino acids 28 to 161 (134 residues), 139.6 bits, E=9.1e-45
• PF13537: GATase_7
  • amino acids 48 to 166 (119 residues), 153.6 bits, E=3.6e-49
• PF00733: Asn_synthase
  • amino acids 243 to 619 (377 residues), 281.3 bits, E=2.7e-87

Best Hits

KEGG orthology group: None (inferred from 51% identity to dma:DMR_42790)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Protein Sequence (638 amino acids)

>MPMX19_06654 Putative asparagine synthetase [glutamine-hydrolyzing] (Azospirillum sp. SherDot2)
MCGFAGFAGPGGERDLAVMTSIIRHRGPDDDGVHIDTDGALAVHLGFRRLAIIDIEGGHQ
PMATAEGDLVIVYNGEIYNAPELRRALEQAGHRFVTDHSDTEVLLHGYREWGLDMLERLN
GMFAFCLYDRRRGRLVMGRDRFGKKPLFYAETADGLVFASEATAVLSHPSVPDTLDRTAL
VKYFAYGFIPAPLTAHAAVRKLPGGCSMVYDLASRRLSVSRYWEYRMVVDDQPPGGPDQW
AEELRELLDRAVERRMLSDVPVGFFLSGGIDSAAIVALAARHRDPALMKTFTIGFDEPSF
DESGYAADAARHYGTDHACRTLNLDTAAGMLPELLGRLDDPIADPSILPTHLLSRFARER
VTVALTGDGGDELFAGYDTFDALRTARLYHHAVPRPLHRLAEAVAHRLPRSDRNMSFDFK
LRRALRGLGHRPAHWMPAWLGPASVEEVSRLFGAELTADELYDEADALWRSGASRHDVDR
SIEFYGRFYLGENLLIKADRASMFCSLETRSPFLDRDLVAFVSRLPAGVKLQPNGIRAGT
RKWILKKAMAPLLPPAILSRPKKGFGIPLSRWLRHLPQPPLETAARIGVDGKWLAARWDE
HRSGQADHRGLLWAWTALATALDRPAPAPPLAPMENAA