Protein Info for MPMX19_06634 in Azospirillum sp. SherDot2

Annotation: GDP-L-fucose synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 57% identical to FCL_SINFN: GDP-L-fucose synthase (fcl) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K02377, GDP-L-fucose synthase [EC: 1.1.1.271] (inferred from 75% identity to azl:AZL_f01640)

MetaCyc: 57% identical to GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase (Arabidopsis thaliana col)
GDP-L-fucose synthase. [EC: 1.1.1.271]

Predicted SEED Role

"GDP-L-fucose synthetase (EC 1.1.1.271)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 1.1.1.271)

MetaCyc Pathways

colanic acid building blocks biosynthesis (10/11 steps found)
GDP-L-fucose biosynthesis I (from GDP-D-mannose) (3/3 steps found)
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (8/14 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.271

Use Curated BLAST to search for 1.1.1.271

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (326 amino acids)

>MPMX19_06634 GDP-L-fucose synthase (Azospirillum sp. SherDot2)
MVLKVRTESLFSLEGKRVWVAGHRGMAGAAVVRRLEREPCDLLTVGRDRVDLRRQEQVED
WMAEAKPDVVFVAAATVGGILANSTRPAEFLYDNLAIETNIIHGAYRTAVKKLVFLGSSC
IYPRLAEQPMREDALLTGPLEPTNEWYAIAKIAGIKMCQAYRRQYGCDFISAMPTNLYGL
NDNFDLQGGHVAAALLAKIHRAKVEGLPFVELWGTGAPRREFLFADDLADGLVFLARHYS
GEPHVNVGTGSEVSIRELAELIRDVVGYEGEFRYDTSKPDGSPRKLMDVSRMSGMGWTAP
TSLRDGFATTYRWFLETVDRGGLRGI