Protein Info for MPMX19_06398 in Azospirillum sp. SherDot2

Annotation: Silver exporting P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 799 transmembrane" amino acids 143 to 164 (22 residues), see Phobius details amino acids 177 to 196 (20 residues), see Phobius details amino acids 208 to 233 (26 residues), see Phobius details amino acids 247 to 266 (20 residues), see Phobius details amino acids 401 to 423 (23 residues), see Phobius details amino acids 429 to 451 (23 residues), see Phobius details amino acids 745 to 767 (23 residues), see Phobius details amino acids 773 to 792 (20 residues), see Phobius details PF04945: YHS" amino acids 31 to 76 (46 residues), 49.5 bits, see alignment 1.1e-16 PF19335: HMBD" amino acids 98 to 124 (27 residues), 52.2 bits, see alignment (E = 1.4e-17) TIGR01511: copper-translocating P-type ATPase" amino acids 191 to 795 (605 residues), 592.1 bits, see alignment E=2.5e-181 TIGR01525: heavy metal translocating P-type ATPase" amino acids 212 to 794 (583 residues), 634.6 bits, see alignment E=3.7e-194 TIGR01494: HAD ATPase, P-type, family IC" amino acids 255 to 764 (510 residues), 297.8 bits, see alignment E=2.3e-92 PF00122: E1-E2_ATPase" amino acids 284 to 464 (181 residues), 199.2 bits, see alignment E=1.3e-62 PF00702: Hydrolase" amino acids 482 to 699 (218 residues), 131.6 bits, see alignment E=1.5e-41

Best Hits

Swiss-Prot: 65% identical to SILP_SALTM: Silver exporting P-type ATPase (silP) from Salmonella typhimurium

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 68% identity to pzu:PHZ_c1055)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (799 amino acids)

>MPMX19_06398 Silver exporting P-type ATPase (Azospirillum sp. SherDot2)
MNDLHHGHGHQPGHDHSGHHHHAGADDHRTVKDPVCGMMVAPERTAHHAEHDGHSFHFCS
ARCHDKFVAAPEQYLKSEAKLAAPERAGQASPQKGVIYTCPMHPEIRQEGPGNCPICGMA
LEPVGASLEEGPNPELVDMTRRFWIGLVLSVPLLVLEMGSHIPALGLHDLVPPRLSIWIQ
FLLATPVVLWAGWPLLERGWQSFRRRSLNMFSLIALGVGVAYLFSLAATFLPGLFPASFR
GMDGVVAVYYEAAAVITVLVLLGQVLELRAREQTGGAIRALLNLAPKTARRIKESGEDEE
VPLDQVRVGDRLRVRPGDGVPVDGEVLEGGSAVDESMVTGESMPVEKEPGAKVIGGTVNQ
TGALVMRAEKVGSDTMLSRIVTMVAEAQRSRAPIQRMADVVSGYFVPAVILAALLAFAGW
MLWGPAPAFAYALIAAVSVLIIACPCALGLATPMSIMVGVGKGATAGVLIKDAASLERFE
KVDTLVVDKTGTLTEGKPRVTAVVPAAGIDEAELLSLAASLERSSEHPLAAAIVASARER
GLALHEVADFGSVTGKGVVGTVGGREVLLGNAAMMRDRGVALDDLDARADDLRREGATAL
FAAVDGRPGGVIAVADPIKASTPHALETLRADGVRIVMLTGDNRTTAEAVARRLGIDEVE
AEVLPDQKHQVVRKLKAEGRVVAMAGDGVNDAPALAEADVGVAMGTGTEVAIQSAGVTLV
KGDLAGIARARVLSRATMDNIRQNLFLAFVYNALGVPVAAGVFYPLFGWTLSPIIAAAAM
ALSSVSVIGNALRLRMTSL