Protein Info for MPMX19_06375 in Azospirillum sp. SherDot2

Annotation: Zinc/cadmium/lead-transporting P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 753 transmembrane" amino acids 145 to 163 (19 residues), see Phobius details amino acids 169 to 187 (19 residues), see Phobius details amino acids 196 to 214 (19 residues), see Phobius details amino acids 220 to 239 (20 residues), see Phobius details amino acids 368 to 390 (23 residues), see Phobius details amino acids 399 to 423 (25 residues), see Phobius details amino acids 706 to 725 (20 residues), see Phobius details amino acids 730 to 749 (20 residues), see Phobius details PF00403: HMA" amino acids 17 to 60 (44 residues), 53.5 bits, see alignment 5.2e-18 TIGR01512: cadmium-translocating P-type ATPase" amino acids 198 to 506 (309 residues), 319.2 bits, see alignment E=9.2e-99 TIGR01525: heavy metal translocating P-type ATPase" amino acids 198 to 748 (551 residues), 586.4 bits, see alignment E=1.5e-179 TIGR01511: copper-translocating P-type ATPase" amino acids 216 to 748 (533 residues), 415.4 bits, see alignment E=8.7e-128 TIGR01494: HAD ATPase, P-type, family IC" amino acids 221 to 725 (505 residues), 269.2 bits, see alignment E=1.1e-83 PF00122: E1-E2_ATPase" amino acids 249 to 429 (181 residues), 179.4 bits, see alignment E=1e-56 PF00702: Hydrolase" amino acids 445 to 660 (216 residues), 125.8 bits, see alignment E=5.9e-40 PF08282: Hydrolase_3" amino acids 631 to 681 (51 residues), 22.8 bits, see alignment 1.4e-08

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 89% identity to azl:AZL_a07690)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (753 amino acids)

>MPMX19_06375 Zinc/cadmium/lead-transporting P-type ATPase (Azospirillum sp. SherDot2)
MNANDETTPTDWQRTRFRVGGMDCGSCAAKIETALRRVPGVHEVRVSVPNGTMTVTHDPS
TGGDAITAPVSRLGYRTGPAEVLTTAGVAAERSDSMDACCGYGHSDSLDRDNARNQAHGH
AHGADEWSDPALGELPWWRRPKAQLALASGLALGVAYVVGALVPSVSPWAFLLAMAVGLV
PIARRAFAAAGAGTPFSIEMLMTIAAVGAVVIGAVEEATAVVFLFLVGELLEGVAAGRAR
ASIQGLIGLMPDSALVEGDGGVRTMPASGLQVGAVILVRPGDRVAADGVILSGDSALDEA
PVTGESVPKRKGEGDRVFAGTINGDGTLRVRVSATAKDNTIARVVRLVEEAQESKAPTER
FIDRFSRWYTPGVVVVASLVAVLPPLLAGGIWGEWIYKGLAVLLIGCPCALVISTPAAIA
AGLSNGARRGLLMKGGAVLEALGRITTVAFDKTGTLTEGKPRVTDVIAFTGGEQEVLARA
ASLEAGSSHPLAKAILAAADDAGLTVGPASDTGALTGKGVRGRLDGLELALVSPRAAGER
TPLTPEQADRVAALNGEGKTVSVLLVDGRLSGLLAMRDEPRADAQAGIAALREAGIRSLM
VTGDNARTARAVADRLGVEPHAELLPEDKQRIVRDLQARGERVAKVGDGINDAPALAAAD
VGVAMGGGTDVALETADAAALHARVGDVAAMVHLSRRTMANIRQNIVLALGLKAVFLVTT
VVGITGLWPAILADTGATVLVTANALRLLSLRP